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Fig. 2 | Virology Journal

Fig. 2

From: Classical swine fever virus NS5A protein changed inflammatory cytokine secretion in porcine alveolar macrophages by inhibiting the NF-κB signaling pathway

Fig. 2

Changes of the RIG-I signaling pathway in CSFV NS5A-transfected cells. In PAMs, 1 μg CSFV NS5A was transfected for 24 h, then cells were untreated or treated with 100 μg/ml poly(I:C). After 24 h culture, extracts of circa 20 μg total cells were prepared and subjected to Western Blotting with antibodies specific for NS5A (a), RIG-I, MDA5 and IPS-1 in PAMs (b). Anti-β-actin was served as an internal control. Band ratio of RIG-I (c), MDA5 (d) and IPS-1 (e) was analyzed using Image J software. The experiment was repeated three times and the figure here shows a representative experiment. An asterisk indicates a statistically significant difference from uninfected cells, *P < 0.05 and **P < 0.01

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