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Fig. 1 | Virology Journal

Fig. 1

From: Classical swine fever virus NS5A protein changed inflammatory cytokine secretion in porcine alveolar macrophages by inhibiting the NF-κB signaling pathway

Fig. 1

CSFV NS5A protein attenuated the inflammatory cytokine production induced by poly(I:C). PAMs were transfected with plasmid expressing NS5A (a). At indicated times, total cell lysates were immunoblotted with rabbit anti-CSFV sera and analyzed by Western Blot. To determine the changes of the inflammatory cytokine production in PAMs, at 24 h following transfection of 1 μg CSFV NS5A, cells were untreated or treated with 100 μg/mL poly(I:C), and cultured for 24 h. Then cell culture supernatants were collected to analyze protein expression of inflammatory cytokines IL-1β (b), IL-6 (c) and TNF-α (d) by ELISA. Data are expressed as mean ± SEM. Representative results are shown of one of three separate experiments. An asterisk indicates a statistically significant difference from uninfected cells, *P < 0.05 and **P < 0.01

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