- Open Access
Distribution of sialic acid receptors and experimental infections with different subtypes of influenza A viruses in Qinghai-Tibet plateau wild pika
- Yan Li†1,
- Haixia Xiao†2,
- Chaobin Huang3,
- Haigang Sun4,
- Laixing Li5,
- Jingliang Su4,
- Juncai Ma6,
- Di Liu6,
- Han Wang1,
- Wenjun Liu1,
- George F Gao1, 2, 4, 7, 8,
- Xiangdong Li3Email author and
- Jinghua Yan1Email author
© Li et al.; licensee BioMed Central. 2015
- Received: 23 September 2014
- Accepted: 27 March 2015
- Published: 14 April 2015
The plateau pika (Ochotona curzoniae) is a small rabbit-like mammal that lives at high altitudes in the Qinghai-Tibet plateau and is in close contact with birds. Following the outbreak of highly pathogenic avian influenza (HPAI) H5N1 during 2005 in the migratory birds of Qinghai Lake, two clades of H5N1 have been found in pikas. However, the influenza virus receptor distribution in different tissues of this animal and its susceptibility to influenza A viruses have remained unclear.
The sialic acid receptor distribution tropism in pika was investigated using fluorescent Sambucus nigra and biotinylated Maackia amurensis I and II. Furthermore, the replication of three influenza A viruses H1N1, H3N2, and H5N1 in this animal was examined by immunohistochemistry and RT-PCR. Morphological and histopathological changes caused by infection were also analyzed with hematoxylin and eosin (H & E) staining.
Human influenza virus-recognizing SAα2,6Gal receptors are widely expressed in the lung, kidney, liver, spleen, duodenum, ileum, rectum, and heart, whereas avian influenza virus-recognizing SAα2,3Gal receptors are strongly expressed in the trachea and lung of pika. M1 could be detected in the lungs of pikas infected with H1N1, H3N2, and H5N1 by either immunostaining or RT-PCR, and in the brain of H5N1-infected pikas. Additionally, three subtypes of influenza A viruses were able to infect pika and caused varying degrees of pneumonia with epithelial desquamation and alveolar inflammatory cell infiltration. Slight pathological changes were observed in H1N1-infected lungs. A few small bronchi and terminal bronchioles were infiltrated by lymphocytic cells in H3N2-infected lungs. In contrast, serious lung damage, such as alveolar capillary hyperemia, edema, alveolar collapse, and lymphocytic infiltrations was observed in H5N1-infected group. Furthermore, neural system changes were present in the brains of H5N1-infected pikas.
SAα2,6Gal receptors are extensively present in many of the tissues and organs in wild plateau pika, whereas SA2,3Gal-linked receptors are dominant on the tracheal epithelial cells. H1N1, H3N2, and H5N1 were able to infect pika and caused different degrees of pathogenic changes in the lungs. Altogether, these results suggest that wild pika has the potential to be a host for different subtypes of influenza A viruses.
- Sialic acid receptors
- Experimental infection
- Influenza A viruses
- Wild plateau pika
- Pathogenic changes
Influenza A viruses are important pathogens in the poultry industry and also pose a large threat to public health. Sixteen hemagglutinin (HA) subtypes and nine neuraminidase (NA) subtypes of influenza A viruses are found in waterfowl , which are considered the natural reservoir of influenza A viruses. Moreover, two novel bat-derived subtypes of influenza A viruses, H17N10 and H18N11, were recently discovered respectively in little yellow-shouldered bats (Sturnira lilium, family Phyllostomidae) and flat-faced fruit bats (Artibeus planirostris) by sequencing analyses [2-4]. However, as yet, those two novel influenza viruses lack classical functions for the viral envelope proteins, the HA cannot bind to any traditional influenza virus receptors, and NA loses the neuraminidase activity to remove sialic acid [3-8]. The discovery of influenza-like viruses in bats has highlighted the concerns of the scientific community over a possible broader host range of the virus.
As stated above, migratory and wild birds (in particular, waterfowl) are generally considered to be the natural hosts for all known subtypes of influenza A viruses, with some subtypes being more adapted to infect humans, birds, pigs, horses, and other animals. Certain subtypes of influenza A viruses that have adapted to infect humans are currently circulating among the human population worldwide, e.g., the H1N1 and H3N2 viruses. Whereas some other HA subtypes of avian influenza virus, such as H5, H6, H7, H9, and H10, have crossed the interspecies barrier and have acquired the ability to infect humans but lack the ability to circulate among humans. Human infections with such avian influenza viruses have ranged from mild symptoms (e.g., conjunctivitis and influenza-like illness) to severe disease, including deaths [9-18]. Worryingly, HPAI virus H5N1 and the low pathogenic avian influenza (LPAI) A virus H7N9 have caused a great number of human infections and deaths. According to the records from the World Health Organization (WHO), H5N1 influenza has caused 650 human infections, with 386 confirmed deaths, from to 2003 to January 24, 2014 . The novel H7N9 virus that emerged in March 2013 has caused 419 infections and 127 deaths until July, 2014 .
Waterfowl and shorebirds have historically been considered the primary natural reservoir for influenza viruses, allowing their circulation with low pathogenicity among the flock. However, the outbreak of H5N1HPAI at Qinghai Lake astonished the world with the death of over 6,000 migratory birds in 2005 [21,22]. Since 2005, H5N1 has spread, following the migration routes of migratory birds, to Europe and some parts of Africa, and has caused severe poultry outbreaks and human infections . In addition, H5N1 has been identified in the plateau pikas , which are found at the Qinghai-Tibet plateau and share their local environment with a plethora of wild birds. Consequently, concerns have been raised as to whether pika is a novel natural host of H5N1 and/or other subtypes of influenza viruses.
A major determinant of infection is the presence of sialosaccharide receptors on the host cell surface to which viral HA can bind. Avian influenza viruses preferentially bind to sialic acid (SA) receptors that are linked to galactose by an α 2,3 linkage (SAα2,3Gal), while human and classical swine viruses show preference for receptors with an α 2,6 linkage (SAα2,6Gal) [25,26]. Distribution tropisms of receptors among different hosts could account for variation in their susceptibility to infection by influenza viruses. The distribution of the influenza virus receptors SAα2,3Gal and SAα2,6Gal has been assessed in several avian and mammal species, and the distribution patterns vary among different species [26-35]. As an important member of the Qinghai-Tibet plateau eco-system, plateau pika was found to be the host of H5N1 influenza viruses . However, little is known about influenza virus receptor distribution in this animal and the pathogenicity caused by H5N1 infection. Thus, we explored the influenza virus receptor distribution in different organs of pika and investigated the susceptibility of the plateau pika to a variety of subtypes of influenza A viruses (including H1N1, H3N2, and H5N1) in this study.
Distribution of SAα2,3Gal and SAα2,6Gal receptors in the different organs of pika
To evaluate the potential role of receptor distribution in the susceptibility of pika to influenza virus infection, we investigated the distributions of SAα2,3Gal and SAα2,6Gal receptors in various organs, such as the trachea, lung, duodenum, ileum, rectum, heart, liver, kidney, brain, and spleen, by using biotinylated Maackia amurensis (MAA) lectin (red) I (MAAI) and II (MAAII) for SAα2,3Gal binding and FITC-labeled Sambucus nigra (SNA) lectin (green) for SAα2,6Gal binding. To eliminate the non-specific staining of MAAI, MAAII and SNA lectins, we used human trachea exclusively expressing SAα2,6Gal receptor and duck duodenum with only SAα2,3Gal expression to determine the background levels of lectin staining. It was clear that these three lectins displayed very low background staining (Additional file 1: Figure S1).
The expression patterns of avian and human receptors were also defined in the kidney, as kidney cell lines are usually utilized for influenza viruses’ isolation and propagation. The MAAI, MAAII, and SNA staining revealed that both avian and human receptors could be detected in the kidney of pika, but MAAII and SNA demonstrated much stronger staining in the epithelial cells from the glomeruli and proximal tubules. In contrast, SAα2,3Gal rather than SAα2,6Gal receptors were present in the epithelial cells from the distal tubules (Figure 1A-f and B-f).
In agreement with the findings in mouse (Mus musculus) , both SAα2,3Gal and SAα2,6Gal receptors were rare in the brain cells from pika (Figure 1A-g and B-g). In the liver, the SAα2,6Gal and SAα2,3Gal stained with MAAII lectin were predominant on the hepatocytes and sinusoid, while weak SAα2,3Gal signal stained with MAAI was found in the liver sinusoid, as well as on the membrane of Kupffer cells (Figure 1A-h and B-h).
In the heart, MAAI, MAAII and SNA displayed very strong staining in the cardiac myocytes (Figure 1A-i and B-i), suggesting that both α2,6Gal and α2,3Gal are widely expressed in the heart. The dominant expression of both avian and human receptors makes heart a very susceptible target for avian and human influenza viruses.
In the spleen, MAAI, MAAII, and SNA staining was detected in both the red and white pulps, but the SAα2,6Gal signal was much more intense. A similar distribution pattern was also found in the lymphatic sheath around the artery in the white pulp regions (Figure 1A-j and B-j).
Pika could be infected with H1, H3, and H5 three subtypes of influenza A viruses
Detection of viruses among the infected pikas with RT-PCR analysis
No. of pika
A/great black-headed gull/Qinghai/1/2009
Pathological changes in the organs and tissues of influenza viruses-infected pika
Following the outbreak of severe H5N1 HPAI in wild birds [21,22], plateau pika infections with two distinct lineages, a mixed Vietnam H5N1 virus lineage and a wild bird Qinghai-like H5N1 virus lineage, were reported in 2007 around Qinghai Lake . Considering that wild birds share the same habitats with pika and that some small birds often enter into pika caves for feeding, critical questions were raised concerning whether influenza virus is highly pathogenic to pika and whether pika could act as an influenza virus-carrier. To answer these questions, we first investigated the influenza virus receptor distribution in plateau pika. One interesting finding was that SAα2,6Gal linked saccharide receptors are present in high amounts in all of the tested organs and tissues, especially in the lungs. The wide presence of SAα2,6Gal linked receptors may facilitate the replication of the human origin influenza A viruses in pika. It also suggests that pika could be more important as an intermediate host for the generation of influenza viruses with increased binding affinity to SAα2,6Gal and SAα2,3Gal receptors.
We also demonstrated that pika could be experimentally infected with various subtypes of influenza A viruses, e.g., human viruses H1N1, H3N2, and the avian virus H5N1. However, virus replication in the lung tissue and viral infectivity differed among these three subtypes, which is consistent with the predominant receptor expression in the trachea and lung and the receptor preferences of human H1N1 and H3N2 and avian H5N1. Dominant expression of SAα2,3Gal in both the trachea and lung facilitates the infection and replication of H5N1 avian influenza virus. In contrast, SAα2,6Gal was distributed mainly in the lungs rather than in the trachea. This explains why much more viral antigen M1-positive cells were stained in the lung of avian receptor-preferring H5N1-infected pikas, and why a higher infection rate occurred in the avian H5N1-challenged group than those in the human receptor-preferring H1N1 and H3N2 infected ones.
Furthermore, H1N1, H3N2, and H5N1 caused different types of damage in the lungs of infected pikas. Similar to the H5N1-infected rabbits (Oryctolagus cuniculus) , H1N1-and H3N2-infected pikas displayed mild pathological changes with lymphocytic infiltrations in the lung. In contrast, serious damage, such as alveolar capillary hyperemia, edema, alveolar collapse, and lymphocytic infiltrations, were observed in the H5N1-infected group. Different degrees of lung damage were correlated with the number of viral antigen M1-positive cells, as well as viral infectivity.
In contrast to the lung tissue, the presence of avian receptor SAα2,3Gal in the trachea, lung, kidney, and spleen may facilitate H5N1 attachment, which in turn caused the lesions and lymphocytic infiltrations in these tissues. We also demonstrated that avian H5N1 infected the pika central neuron system (CNS) by attacking Purkinje neurons in the cerebellum, which is in consistent with H5N1 infection in ferret (Mustela putorius furo) and crow (Corvus) [36,37]. These results demonstrate that pika is more susceptible to H5N1 avian influenza virus, and the pathogenic response of pika to influenza viruses is subtype- or strain-dependent, though the mechanism involved needs to be further explored.
Taken together, we demonstrated that pika is capable of being an influenza host, though human origin H1N1 and H3N2 viruses had relative low replication efficiency in the lung than avian H5N1. As the influenza virus continues to circulate within the pika population, the influenza viruses that have been found in wild plateau pika are most likely transmitted from wild birds. The presence of SAα2,6Gal and SAα2,3Gal receptors in many organs suggest that pika could act as an efficient virus reservoir and possible mixing vessels. Consequently, we would advise that monitoring of viruses among the wild bird populations in the Qinghai-Tibet plateau should also be extended to monitor the wild pika.
Virus strains and virus amplification
Influenza viruses A/WSN/1933 (H1N1) and A/Jiangxi/262/2005 (H3N2) were amplified in MDCK cells; A/great black-headed gull/Qinghai/1/2009 (H5N1) was propagated in 10 day-old SPF embryonated eggs. Viral titers were determined by plaque assays. Briefly, confluent MDCK cells in 12-well plates were washed twice with PBS and then infected with 500 μl virus in DMEM (GIBCO, Grand Island, NY) at a concentration range from 10−1 to 10−6 for 1 hour (h) at 37°C with gentle shaking. The inocula were then removed, and the cells were washed twice and overlaid with DMEM containing 1% agarose (Promega, Madison, WI) and 5 μg/ml TPCK treated trypsin. After overlays are solidified, the cells were incubated at 37°C with 5% CO2 for 72 hours (hrs). Cells were then fixed with 4% formaldehyde in PBS for 1 h and stained with neutral red at room temperature (RT). All experiments with the H5N1 virus were performed in a biosafety level 3 laboratory.
Lectin detection of SAα2,3Gal and SAα2,6Gal
Sections of various tissues were deparaffinized and rehydrated as described below for H & E staining. After being washed in distilled water for 5 minutes at RT, sections were heat-treated with the antigen-retrieval solution (citrate buffer, pH 6.0) four times, each time for 4 minutes. Before being stained with lectins, sections were blocked with 10% horse serum (GIBCO, Grand Island, NY) for 10 minutes at RT. Tissue sections were incubated with specific FITC-labeled SNA lectin to detect SAα2,6Gal receptor (Vector Laboratories, Burlingame, CA) and biotinylated MAAI and MAAII lectins for SAα2,3Gal (Vector Laboratories, USA ) for 2 hrs at 37°C. After being washed with PBS three times, the sections were incubated with PE-conjugated streptavidin (Sigma, Santa Clara, CA) for 2 hrs at RT. The sections were washed in PBS three times to remove unbound streptavidin and then counterstained with 4,6-diamidino-2-phenylindole (DAPI) (Roche, Switzerland).
Animal infection with influenza A viruses
The pika animals in this experiment were caught in regions around Haibei Alpine Grassland Ecosystem Research Station in Qinghai Province, China. Sera collected from pikas via the lateral tail veins were tested by HI and neutralization assays with H1, H3, and H5 influenza viruses to rule out influenza virus sero-positive animals. Female adult pikas with body masses of approximately 100-170 g were selected and kept in captivity for 7 days to adapt to the environment before being infected with influenza viruses. Food and water were provided every day. The animals were anesthetized with pentobarbital sodium salt (Sigma, 40 mg/kg) via intraperitoneal injection and intranasally infected with 7.0 × 104 pfu of H5N1, H1N1, or H3N2 virus. Five days post virus infection, pikas were sacrificed, and tissues, including the heart, liver, spleen, half of the lung, trachea, kidney, brain, and intestine, were collected and stored in neutral formalin; the other half of the lungs were frozen in liquid nitrogen for RNA extraction.
The Ethics Committee for animal experimentation of the Institute of Microbiology, Chinese Academy of Sciences, approved all of the animal experiments (Registry No.CASPMI2008001).
Immunohistochemistry (IHC) detection of influenza A virus M1 protein
Immunohistochemical staining was performed as described below. Reagents used in this study were described as follows: mouse anti-M1 monoclonal antibody (1:200 dilution, a gift from Professor Wenjun Liu, Institute of Microbiology, CAS.), biotinylated goat-anti-mouse secondary antibody (1:200 dilution, Vector Laboratories, USA), and strepavidin-conjugated HRP (1:200 dilution, Jackson Immuno Research, West Grove, PA).
Viral RNA extraction and RT-PCR
To identify infection in experimental pikas, viral RNA from the lung of each pika was extracted as described in the RNeasy Mini kit (Qiagen, Hilden, Germany). Briefly, 30 mg lung tissue was disrupted and homogenized in 600 μl RLT buffer supplied in the kit, and the nucleic acids were eluted with 30 μl nuclease-free water. Lungs from uninfected pikas were used as the negative control. Viral cDNA was immediately obtained using a Reverse Transcription System kit (Promega, Madison, WI) with primer Uni12 5′-AGCAAAAGCAGG-3′. Amplification of the M segment was performed with primers UMF 5′-TATTCGTCTC AGGGAGCAAAAGCAGGTAG-3′, and UMR5′-ATATCGTCTCGTATTAGTAGAAACAAGGTAGTTTTT-3′.
Morphological and histological analyses
Tissues collected from pikas were dehydrated with increasing concentrations of ethanol, vitrified by dimethylbenzene, and then embedded in paraffin. After being embedded, the tissues were sectioned with a rotary microtome (Hestion ERM3000, China). Sections (5 μm-thicknesses) were deparaffinized in dimethylbenzene, rehydrated using graded ethanol, and stained with H & E for histopathological examination. Before being mounted, sections were dehydrated and vitrified. Mounted slides were observed under a fluorescence microscope (IX71 Olympus Co., Ltd., Tokyo) and photographed with a Nikon DXM1200F (Nikon Co., Tokyo).
This work was supported by the Ministry of Science and Technology of China (MOST) 973 Project (Grant No. 2010CB530303), Youth Innovation Promotion Association, CAS, and US Civilian Research & Development Foundation from the National Institute of Allergy and Infectious Diseases (No. CHB1-31057-BE-11). George F. Gao is a leading principal investigator of the National Natural Science Foundation of China (NSFC) Innovative Research Group (Grant No. 81321063). We thank Joel Heywood and Guangwen Lu for critical reading and English editing.
- Kim JK, Negovetich NJ, Forrest HL, Webster RG. Ducks: the “Trojan horses” of H5N1 influenza. Influenza Other Respir Viruses. 2009;3:121–8.View ArticlePubMed CentralPubMedGoogle Scholar
- Tong S, Li Y, Rivailler P, Conrardy C, Castillo DA, Chen LM, et al. A distinct lineage of influenza A virus from bats. Proc Natl Acad Sci U S A. 2012;109:4269–74.View ArticlePubMed CentralPubMedGoogle Scholar
- Tong S, Zhu X, Li Y, Shi M, Zhang J, Bourgeois M, et al. New world bats harbor diverse influenza A viruses. PLoS Pathog. 2013;9:e1003657.View ArticlePubMed CentralPubMedGoogle Scholar
- Wu Y, Wu Y, Tefsen B, Shi Y, Gao GF. Bat-derived influenza-like viruses H17N10 and H18N11. Trends Microbiol. 2014;22:183–91.View ArticlePubMedGoogle Scholar
- Sun X, Shi Y, Lu X, He J, Gao F, Yan J, et al. Bat-derived influenza hemagglutinin H17 does not bind canonical avian or human receptors and most likely uses a unique entry mechanism. Cell Rep. 2013;3:769–78.View ArticlePubMedGoogle Scholar
- Zhu X, Yu W, McBride R, Li Y, Chen LM, Donis RO, et al. Hemagglutinin homologue from H17N10 bat influenza virus exhibits divergent receptor-binding and pH-dependent fusion activities. Proc Natl Acad Sci U S A. 2013;110:1458–63.View ArticlePubMed CentralPubMedGoogle Scholar
- Li Q, Sun X, Li Z, Liu Y, Vavricka CJ, Qi J, et al. Structural and functional characterization of neuraminidase-like molecule N10 derived from bat influenza A virus. Proc Natl Acad Sci U S A. 2012;109:18897–902.View ArticlePubMed CentralPubMedGoogle Scholar
- Zhu XY, Yang H, Guo Z, Yu WL, Carney PJ, Li Y, et al. Crystal structures of two subtype N10 neuraminidase-like proteins from bat influenza A viruses reveal a diverged putative active site. Proc Natl Acad Sci U S A. 2012;109:18903–8.View ArticlePubMed CentralPubMedGoogle Scholar
- Subbarao K, Klimov A, Katz J, Regnery H, Lim W, Hall H, et al. Characterization of an avian influenza A (H5N1) virus isolated from a child with a fatal respiratory illness. Science. 1998;279:393–6.View ArticlePubMedGoogle Scholar
- Beigel JH, Farrar J, Han AM, Hayden FG, Hyer R, de Jong MD, et al. Avian influenza A (H5N1) infection in humans. N Engl J Med. 2005;353:1374–85.View ArticlePubMedGoogle Scholar
- Yuan J, Zhang L, Kan X, Jiang L, Yang J, Guo Z, et al. Origin and molecular characteristics of a novel 2013 avian influenza A(H6N1) virus causing human infection in Taiwan. Clin Infect Dis. 2013;57:1367–8.View ArticlePubMedGoogle Scholar
- Skowronski DM, Tweed SA, Petric M, Booth T, Li Y, Tam T. Human illness and isolation of low-pathogenicity avian influenza virus of the H7N3 subtype in British Columbia, Canada. J Infect Dis. 2006;193:899–900. author reply 900–891.View ArticlePubMedGoogle Scholar
- Eames KT, Webb C, Thomas K, Smith J, Salmon R, Temple JM. Assessing the role of contact tracing in a suspected H7N2 influenza A outbreak in humans in Wales. BMC Infect Dis. 2010;10:141.View ArticlePubMed CentralPubMedGoogle Scholar
- Fouchier RA, Schneeberger PM, Rozendaal FW, Broekman JM, Kemink SA, Munster V, et al. Avian influenza A virus (H7N7) associated with human conjunctivitis and a fatal case of acute respiratory distress syndrome. Proc Natl Acad Sci U S A. 2004;101:1356–61.View ArticlePubMed CentralPubMedGoogle Scholar
- Peiris M, Yuen KY, Leung CW, Chan KH, Ip PL, Lai RW, et al. Human infection with influenza H9N2. Lancet. 1999;354:916–7.View ArticlePubMedGoogle Scholar
- Arzey GG, Kirkland PD, Arzey KE, Frost M, Maywood P, Conaty S, et al. Influenza virus A (H10N7) in chickens and poultry abattoir workers, Australia. Emerg Infect Dis. 2012;18:814–6.View ArticlePubMed CentralPubMedGoogle Scholar
- Chen H, Yuan H, Gao R, Zhang J, Wang D, Xiong Y, et al. Clinical and epidemiological characteristics of a fatal case of avian influenza A H10N8 virus infection: a descriptive study. Lancet. 2014;383:714–21.View ArticlePubMedGoogle Scholar
- Zhang T, Bi Y, Tian H, Li X, Liu D, Wu Y, et al. Human infection with influenza virus A(H10N8) from live poultry markets, China, 2014. Emerg Infect Dis. 2014;20:2076–9.PubMed CentralPubMedGoogle Scholar
- World Health Organization. Cumulative number of confirmed human cases for avian influenza A (H5N1) reported to WHO, 2003–2014. http://www.who.int/influenza/human_animal_interface/EN_GIP_20140124CumulativeNumberH5N1cases.pdf; 24 January 2014.
- World Health Organization. Confirmed human cases of avian influenza A (H7N9) reported to WHO. http://www.who.int/influenza/human_animal_interface/influenza_h7n9/18_reportwebh7n9number_20140714.pdf?ua=1; 14 July 2014.
- Liu J, Xiao H, Lei F, Zhu Q, Qin K, Zhang XW, et al. Highly pathogenic H5N1 influenza virus infection in migratory birds. Science. 2005;309:1206.View ArticlePubMedGoogle Scholar
- Chen H, Smith GJ, Zhang SY, Qin K, Wang J, Li KS, et al. Avian flu: H5N1 virus outbreak in migratory waterfowl. Nature. 2005;436:191–2.View ArticlePubMedGoogle Scholar
- World Health Organization. H5N1 highly pathogenic avian influenza: Timeline of major events. http://www.who.int/influenza/human_animal_interface/H5N1_avian_influenza_update20121210.pdf; 10 December 2013.
- Zhou JY, Sun WB, Wang JH, Guo JQ, Yin W, Wu NP, et al. Characterization of the H5N1 highly pathogenic avian influenza virus derived from wild pikas in China. J Virol. 2009;83:8957–64.View ArticlePubMed CentralPubMedGoogle Scholar
- Gagneux P, Cheriyan M, Hurtado-Ziola N, van der Linden EC, Anderson D, McClure H, et al. Human-specific regulation of alpha 2-6-linked sialic acids. J Biol Chem. 2003;278:48245–50.View ArticlePubMedGoogle Scholar
- Matrosovich MN, Matrosovich TY, Gray T, Roberts NA, Klenk HD. Human and avian influenza viruses target different cell types in cultures of human airway epithelium. Proc Natl Acad Sci U S A. 2004;101:4620–4.View ArticlePubMed CentralPubMedGoogle Scholar
- Ibricevic A, Pekosz A, Walter MJ, Newby C, Battaile JT, Brown EG, et al. Influenza virus receptor specificity and cell tropism in mouse and human airway epithelial cells. J Virol. 2006;80:7469–80.View ArticlePubMed CentralPubMedGoogle Scholar
- Kuchipudi SV, Nelli R, White GA, Bain M, Chang KC, Dunham S. Differences in influenza virus receptors in chickens and ducks: Implications for interspecies transmission. J Mol Genet Med. 2009;3:143–51.View ArticlePubMed CentralPubMedGoogle Scholar
- Ning ZY, Luo MY, Qi WB, Yu B, Jiao PR, Liao M. Detection of expression of influenza virus receptors in tissues of BALB/c mice by histochemistry. Vet Res Commun. 2009;33:895–903.View ArticlePubMedGoogle Scholar
- Jayaraman A, Chandrasekaran A, Viswanathan K, Raman R, Fox JG, Sasisekharan R. Decoding the distribution of glycan receptors for human-adapted influenza A viruses in ferret respiratory tract. PLoS One. 2012;7:e27517.View ArticlePubMed CentralPubMedGoogle Scholar
- Kim M, Yu JE, Lee JH, Chang BJ, Song CS, Lee B, et al. Comparative analyses of influenza virus receptor distribution in the human and mouse brains. J Chem Neuroanat. 2013;52:49–57.View ArticlePubMedGoogle Scholar
- Zeng H, Goldsmith CS, Maines TR, Belser JA, Gustin KM, Pekosz A, et al. Tropism and infectivity of influenza virus, including highly pathogenic avian H5N1 virus, in ferret tracheal differentiated primary epithelial cell cultures. J Virol. 2013;87:2597–607.View ArticlePubMed CentralPubMedGoogle Scholar
- Trebbien R, Larsen LE, Viuff BM. Distribution of sialic acid receptors and influenza A virus of avian and swine origin in experimentally infected pigs. Virol J. 2011;8:434.View ArticlePubMed CentralPubMedGoogle Scholar
- Shinya K, Ebina M, Yamada S, Ono M, Kasai N, Kawaoka Y. Avian flu: influenza virus receptors in the human airway. Nature. 2006;440:435–6.View ArticlePubMedGoogle Scholar
- Nelli RK, Kuchipudi SV, White GA, Perez BB, Dunham SP, Chang KC. Comparative distribution of human and avian type sialic acid influenza receptors in the pig. BMC Vet Res. 2010;6:4.View ArticlePubMed CentralPubMedGoogle Scholar
- Plourde JR, Pyles JA, Layton RC, Vaughan SE, Tipper JL, Harrod KS. Neurovirulence of H5N1 infection in ferrets is mediated by multifocal replication in distinct permissive neuronal cell regions. PLoS One. 2012;7:e46605.View ArticlePubMed CentralPubMedGoogle Scholar
- Tanimura N, Tsukamoto K, Okamatsu M, Mase M, Imada T, Nakamura K, et al. Pathology of fatal highly pathogenic H5N1 avian influenza virus infection in large-billed crows (Corvus macrorhynchos) during the 2004 outbreak in Japan. Vet Pathol. 2006;43:500–9.View ArticlePubMedGoogle Scholar
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.