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- Open Access
Replication kinetics of duck enteritis virus UL16 gene in vitro
© He et al.; licensee BioMed Central Ltd. 2012
- Received: 19 November 2011
- Accepted: 23 October 2012
- Published: 21 November 2012
The function and kinetics of some herpsvirus UL16 gene have been reported. But there was no any report of duck enteritis virus (DEV) UL16 gene.
The kinetics of DEV UL16 gene was examined in DEV CHv infected duck embryo fibroblasts (DEFs) by establishment of real-time quantitative reverse transcription polymerase chain reaction assay (qRT-PCR) and western-blotting. In this study, UL16 mRNA was transcript at a low level from 0–18 h post-infection (p.i), and peaked at 36 h p.i. It can’t be detected in the presence of acyclovir (ACV). Besides, western-blotting analysis showed that UL16 gene expressed as an apparent 40-KDa in DEV infected cell lysate from 12 h p.i, and rose to peak level at 48 h p.i consistent with the qRT-PCR result.
These results provided the first evidence of the kinetics of DEV UL16 gene. DEV UL16 gene was a late gene and dependent on viral DNA synthesis.
- Duck Enteritis Virus
- UL16 Gene
- Quantitative Reverse Transcription Polymerase Chain
- UL16 Protein
- Duck Embryo Fibroblast
Duck enteritis virus (DEV), caused duck viral enteritis (DVE), which was an acute, contagious and widespread disease in the family anatidae of the world, known as Duck plague virus (DPV), is a member of subfamily Alphaherpesvirinae of the family Herpesviridae, and has been assigned into Mardivirus genus [1, 2]. DEV is composed of four structures, linear double strand DNA, icosahedral capsid, tegument and envelope. The DEV CHv strain complete genomic sequence and gene library has been constructed in our laboratory . To date, a few of DEV genes of the kinetics has been identified and reported, such as US3 , UL31 , UL38 , UL45 , UL51 , and UTPase . There was no report of DEV UL16 gene yet. In this study, real-time quantitative reverse transcription polymerase chain reaction assay (qRT-PCR) and western-blotting were used to determine the kinetics of DEV UL16 gene.
In this work, we have firstly presented the kinetics of DEV UL16 gene. The productions of DEV UL16 gene accumulated at the late times of infection and couldn’t be detected in the presence of ACV, suggesting that the UL16 gene belonged to γ2 gene and might encode a structural protein which takes part in virion assembly, budding, and egress. The study is in accord with the earlier researches of HSV-1 UL16, HSV-2 UL16 and HCMV UL94 genes. All these results may provide some insights for further studies on the function of DEV UL16 gene.
The research was supported by grants from China 973 program(2011CB111606), Changjiang Scholars and Innovative Research Team in University (PCSIRT0848) and China Agricultural Research System (CARS-43-8).
- Fadly AM, Glisson JR, McDougald LR, Nolan L, Swayne DE: Duck Virus Enteritis. 2008, Wiley-BlackwellSaif YM, Diseases of Poultry American, 384-393.Google Scholar
- King A, Lefkowita E, Adams MJ: Virus taxonomy: Ninth report of the International Committee on Taxonomy of Viruses. Elsevier. 2011, 111-114.Google Scholar
- Wu Y, Cheng AC, Wang MS, Yang Q, Zhu DK, Jia RY, Chen S, Zhou Y, Wang XY, Chen XY: Complete genomic sequence of chinese virulent duck enteritis virus CHv stain. J Virol. 2012, 10: 5965-5965.View ArticleGoogle Scholar
- Xin HY, Cheng AC, Wang MS, Jia RY, Shen CJ, Chang H: Identification and Characterization of a Duck Enteritis Virus US3-Like Gene. Avian Dis. 2009, 53: 363-369. 10.1637/8643-020409-Reg.1.PubMedView ArticleGoogle Scholar
- Xie W, Cheng AC, Wang MS, Chang H, Zhu DK, Luo QH: Expression and characterization of the UL31 protein from duck enteritis virus. Virol J. 2009, 6: 19-10.1186/1743-422X-6-19.PubMedPubMed CentralView ArticleGoogle Scholar
- Xiang J, Ma GP, Zhang SC, Cheng AC, Wang MS, Zhu DK, Jia RY, Luo QH, Chen ZL, Chen XY: Expression and intracellular localization of duck enteritis virus pUL38 protein. Virol J. 2010, 7: 162-10.1186/1743-422X-7-162.PubMedPubMed CentralView ArticleGoogle Scholar
- Shen AM, Ma GP, Cheng AC, Wang MS, Luo DD, Lu LT, Zhou T, Zhu DK, Luo QH, Jia RY, Chen ZL, Zhou Y, Chen XY: Transcription phase, protein characteristics of DEV UL45 and prokaryotic expression, antibody preparation of the UL45 des-transmembrane domain. Virol J. 2010, 7: 232-10.1186/1743-422X-7-232.PubMedPubMed CentralView ArticleGoogle Scholar
- Shen CJ, Guo YF, Cheng AC, Wang MS, Zhou Y, Lin D, Xin HY, Zhang N: Identification and characterization of the duck enteritis virus UL51 gene. Arch Virol. 2009, 154: 1061-1069. 10.1007/s00705-009-0407-8.PubMedView ArticleGoogle Scholar
- Zhao LC, Cheng AC, Wang MS, Yuan GP, Jia RY, Zhou DC, Qi XF, Ge H, Sun T: Identification and characterization of duck enteritis virus dUTPase gene. Avian Dis. 2008, 52: 324-331. 10.1637/8169-110607-ResNote.1.PubMedView ArticleGoogle Scholar
- Livak KJ, Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method. Methods. 2001, 25: 402-408. 10.1006/meth.2001.1262.PubMedView ArticleGoogle Scholar
- Park NH, Langston AP, Mclean SL, Albert AM: Therapy of experimental herpes simplex encephalitis with acyclovir in mice. Antimicrob Agents Chemother. 1979, 15: 775-779. 10.1128/AAC.15.6.775.PubMedPubMed CentralView ArticleGoogle Scholar
- Oshima S, Daikoku T, Shibata S, Yamada H, Goshima F, Nishiyama Y: Characterization of the UL16 gene product of herpes simplex virus type 2. Arch Virol. 1998, 143: 863-880. 10.1007/s007050050338.PubMedView ArticleGoogle Scholar
- Nalwanga D, Rempel S, Roizman B, Baines JD: The UL16 gene product of herpes simplex virus 1 is a virion protein that colocalizes with intranuclear capsid proteins. Virology. 1996, 226: 236-242. 10.1006/viro.1996.0651.PubMedView ArticleGoogle Scholar
- He Q, Yang Q, Cheng AC, Wang MS, Xiang J, Zhu DK, Jia RY, Luo QH, Chen ZL, Chen XY: Expression and characterization of Ul16 Gene from duck enteritis virus. Virol J. 2011, 8: 413-10.1186/1743-422X-8-413.PubMedPubMed CentralView ArticleGoogle Scholar
- Wing BA, Lee GC, Huang ES: The human cytomegalovirus UL94 open reading frame encodes a conserved herpes virus capsid/tegument-associated virion protein that is expressed with true late kinetics. J Virol. 1996, 70: 3339-3345.PubMedPubMed CentralGoogle Scholar
- Guo H, Wang L, Peng L, Zhou ZH, Deng H: Open Reading Frame 33 of a Gammaherpesvirus Encodes a Tegument Protein Essential for Virion Morphogenesis and Egress. J Virol. 2009, 83: 10582-10595. 10.1128/JVI.00497-09.PubMedPubMed CentralView ArticleGoogle Scholar
- Baines JD, Roizman B: The open reading frames UL3, UL4, UL10, and UL16 are dispensable for the replication of herpes simplex virus 1 in cell culture. J Virol. 1991, 65: 938-944.PubMedPubMed CentralGoogle Scholar
- Meckes DG, Wills JW: Dynamic interactions of the UL16 tegument protein with the capsid of herpes simplex virus. J Virol. 2007, 81: 13028-13036. 10.1128/JVI.01306-07.PubMedPubMed CentralView ArticleGoogle Scholar
- Weir JP: Regulation of herpes simplex virus gene expression. Gene. 2001, 271: 117-130. 10.1016/S0378-1119(01)00512-1.PubMedView ArticleGoogle Scholar
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