- Open Access
The pandemic (H1N1) 2009 influenza virus is resistant to mannose-binding lectin
© Tokunaga et al; licensee BioMed Central Ltd. 2011
- Received: 20 December 2010
- Accepted: 4 February 2011
- Published: 4 February 2011
Mannose-binding lectin (MBL) is an important component of innate immunity because it promotes bacterial clearance and neutralization of human influenza A viruses. Since a majority of humans have no neutralizing antibody against the pandemic (H1N1) 2009 influenza (pandemic 2009) virus, innate immunity may be crucial and MBL susceptibility may therefore influence viral pathogenesis.
We examined MBL susceptibility of influenza A viruses and observed that the pandemic 2009 virus was resistant to MBL, whereas all seasonal influenza A viruses tested were susceptible. The mortality of mice infected with a seasonal H1N1 influenza virus was evidently enhanced on transient blockage of MBL activity by simultaneous inoculation of mannan, whereas mannan inoculation had no effect on mice infected with a pandemic 2009 virus. This indicates that MBL protects mice against infection with the seasonal virus but not against that with the pandemic 2009 virus.
These results indicate that the pandemic 2009 virus is not susceptible to MBL, an important component of innate immunity.
- Influenza Virus
- MDCK Cell
- Virus Suspension
- Mouse Seron
A novel influenza virus of swine origin, which emerged in North America in 2009, rapidly spread worldwide and caused the influenza pandemic 2009. This virus was classified as type A and subtype H1N1 according to the antigenicity of hemagglutinin (HA) and neuraminidase proteins . Currently, the pandemic 2009 caused due to influenza H1N1 virus has ceased. However, in case of the 1918 Spanish flu pandemic, low mortality was observed at the first wave, followed by a second wave that caused a severe pandemic with high mortality [2, 3]. Whether a second wave of the pandemic (H1N1) 2009 will emerge is difficult to predict. Pathogenesis of the pandemic (H1N1) 2009 influenza (pandemic 2009) virus has not yet been completely elucidated. Pathogenesis of a virus is not only determined by its ability to infect and grow in its host but also by its interaction with host defense mechanisms. Prior to an acquired immune response, especially in case of primary infection with a foreign pathogen, innate immunity is crucial for host defense. Consequently, the susceptibility of a pathogen to an innate immune response inevitably determines its pathogenesis. Human seasonal influenza A viruses are susceptible to mannan-binding protein, also known as mannose-binding lectin (MBL) [4–9], which is an acute protein produced by the liver [10, 11]. Usually, the blood MBL level may not be sufficiently high to directly inhibit initial infection with the influenza virus. However, when MBL production is upregulated in response to inflammation, MBL may restrict the development of viral infection in the host. Thus, it is expected to function as a "brake" towards inhibiting the viral propagation. Therefore, MBL susceptibility of the pandemic 2009 virus must be determined in order to discuss the pathogenesis of this virus and the potential toward causing a severe second wave of pandemic.
MBL susceptibility of the pandemic 2009 virus
Glycosylation site of the HA protein responsible for MBL susceptibility
Contribution of MBL to host defense in mice infected with seasonal or pandemic 2009 viruses
Human influenza A viruses excluding mouse-adapted viruses have a glycosylation site at amino acid 165 of the HA protein . Glycan at amino acid 165 of the H3 HA protein is rich in mannose, and is thus responsible for MBL susceptibility of human H3N2 influenza viruses [4, 7]. The 1918 Spanish flu virus has no glycosylation site around amino acid 165 of the HA protein , and therefore, it might be resistant to MBL. Through reverse genetics, Kobasa et al. substituted the HA protein of a human-origin influenza virus with that of the Spanish flu virus and found that the resulting virus caused severe pneumonia with extensive cellular infiltration in mice . They proposed that the high pathogenicity of this virus was due to an abnormality in the innate immune response of the mice. Accordingly, we suggest that MBL, which is an important component of innate immunity, might fail to neutralize this virus in the mouse lung.
This raises the question as to why the pandemic 2009 virus is not as virulent in humans as the Spanish flu virus. In our experiments with human-origin cell cultures (unpublished data), the growth rate of the pandemic 2009 virus was distinctly lower than that of seasonal H3N2 and H1N1 viruses, suggesting that the pandemic virus may not yet have adapted sufficiently to the human cells. Thus, the pandemic 2009 virus can potentially cause a severe second wave of pandemic, once it acquires such a high growth rate in humans.
Although the advantage of the presence of a mannose-rich glycan at the tip of the HA protein of the virus remains unclear, it may attenuate viral pathogenesis, facilitating patient mobility and thereby contributing to wide dissemination of the virus, even after a large population of humans has obtained immunity against it. By 1933, a descendant of the Spanish flu virus obtained a glycosylation site at amino acid 165 of the HA protein to which a mannose-rich glycan could attach . Since no virus was isolated from humans before 1933, the exact year of this development remains unknown.
In the database of influenza viruses, four pandemic 2009 isolates with the glycosylation motif at amino acid 165 in the HA protein have been reported , which were first isolated in Novouralsk, Russia (November 2009) and later in Orenburg, Russia (March 2010). Both of these regions are located in Central Asia, and the nucleotide sequence homology of HA is >99.6% among the isolates , suggesting that they belong to the same lineage. This lineage may be either extinct or present in an extremely lower number of human populations. Such MBL susceptible viruses may have no competitive advantage in the present situation, wherein a majority of people have no immunity against the virus.
The contribution of MBL to host defense in influenza infection has not yet been completely elucidated. Regarding the innate host defense mechanism in respiratory organ, surfactant protein D (SP-D) and A (SP-A) should be considered as the most potent factors in the defense mechanism [21–23]. Since SP-D is known to show sugar specificity similar to MBL , it would be interesting to examine the susceptibility of the pandemic 2009 virus to SP-D and also to SP-A. These factors will now be of utmost importance towards advancing our understanding of influenza pathogenesis. Future studies including extensive clinical studies are urgently required in this area of research.
The pandemic 2009 influenza virus is not susceptible to MBL, and therefore, the MBL resistance property of this virus may be one of its potential pathogenic factors.
Viruses and cells
Pandemic influenza A/Chiba/1001 and 1007/2009 (H1N1) viruses were provided by Dr. Ogawa T from the Chiba Prefectural Institute of Public Health. The A/Kurashiki/3/2009 (H1N1) virus was isolated in our laboratory. For seasonal influenza viruses, we used A/USSR/90/1977 (H1N1) (provided by Dr. Nobusawa R from the Nagoya City University), A/Okayama/5/2000 (H1N1), and A/Okayama/6/2001 (H3N2) (both isolated in our laboratory). Each virus stock was purified twice by limiting dilutions through MDCK cells grown in a 12-well plate and then propagated once in MDCK cells in a bottle. The passage number of Chiba, Kurashiki, and Okayama strains used in the present study was between 5 and 7. The USSR/77 virus was propagated once in an embryonated chicken egg.
MDCK cells were grown in Eagle's minimum essential medium supplemented with 10% fetal calf serum (FCS). For viral infection, MDCK cells were washed twice with FCS-free medium and then supplemented with fresh DMEM. All viral experiments were performed in a level 2 biosafety laboratory.
Assay of viral infectivity
MDCK cells propagated in a 12-well culture plate were inoculated with virus suspensions and incubated for 1 h at 37°C, and then the culture medium was replaced with DMEM containing 0.6% agarose and 1 μg/ml acetylated trypsin (Sigma, USA). In agarose-containing medium, trypsin-activated progeny viruses infected only neighboring cells and thus formed a plaque. Accordingly, one PFU was considered to correspond to one infectious virus. Two days after virus inoculation, the plaques were stained with anti-influenza A virus guinea pig antiserum (prepared in our laboratory), and peroxidase-conjugated goat anti-guinea pig IgG (Jackson Immuno Research, USA).
Neutralization of viral infectivity with murine MBL
Sera were prepared from 3.5- to 7.5-week-old naive C57BL/10slc or BALB/c mice (SLC Laboratory, Japan). In order to measure the virus neutralizing activity, serum was serially diluted 10-fold with DMEM, and then mixed with the virus suspension. To block MBL activity, the serum was diluted with DMEM containing 0.1 M mannose (Sigma, USA). After incubating the mixtures for 30 min at room temperature, they were inoculated into MDCK cell cultures to determine the remaining infectivity.
Sequence analysis of the region around amino acid 165 in the HA protein
Virus suspension (1.5 ml) was centrifuged at 10,000 × g for 90 min at 4°C, and RNA was extracted from the precipitate using Isogen (Wako Chemicals, Japan). HA cDNA was synthesized from RNA by RT-PCR using specific primers. The PCR product was subjected to agarose gel electrophoresis, and the specific band was excised from the gel and purified using a QIAquick Gel Extraction Kit (Qiagen, Germany). Sequencing was performed using the same primers as those used in RT-PCR. The primers used for the seasonal Okayama/5/2000 strain were huH1-257 (forward, 5'-TAC TGA TTT CCA AGG AGT C-3') and huH1-885 (reverse, 5'-CTT CGC ATC ACA TTT ATC CAT-3'), and those used for the pandemic Chiba/1001/2009 and Kurashiki/3/2009 strains were swH-257 (forward, 5'-CAC TCT CCA CAG CAA GCT C-3') and swH-885 (reverse, 5'-AGT TGT ATT GCA ATC GTG GAC-3'), respectively. The nucleotide sequences were deposited in the DNA Data Bank of Japan (accession number AB568299-301).
Experimental infection of mice with seasonal and pandemic viruses
Female 3.5-week-old C57BL/10slc or BALB/c mice were intranasally inoculated with a 20 μl suspension of the seasonal Okayama/5/2000(H1N1) or pandemic 2009 Chiba/1001/09(H1N1) viruses under anesthesia with sevoflurane. The body weight of each mouse was monitored daily. For histopathological examination, lungs were removed from several mice on day 8 after infection. At the end of the experiment, blood samples were collected from the surviving mice under anesthesia and the anti-influenza antibody titer in the serum was assayed to confirm infection; this analysis was performed even for asymptomatic mice.
To temporarily block MBL activity in mice, soluble mannan (Sigma, USA) was added to the virus suspension at a final concentration of 1% (w/v). Severity scores of the infected mice administrated with and without mannan were statistically compared using Welch's two-tailed t-test. This experiment was approved by the Animal Experiment Committee of Kawasaki Medical School (Approval No. 09-066) and performed in a P2A level laboratory.
We thank Dr. Terada K from the Department of Pediatrics; Ogoh S from Animal Experiment Research Center; Saika Y, Matoba K, Omori S, Dr. Fujii Y, and Dr. Okino T from the Department of Microbiology; Dr. Nishimura H from the Department of Pathology, and the staff from Tissue Electron Microscopic Research Center and Tissue Culture Research Center of Kawasaki Medical School for their help and cooperation. This work was supported by Project Research Grants (20-4070, 21-412) from Kawasaki Medical School.
- Neumann G, Noda T, Kawaoka Y: Emergence and pandemic potential of swine-origin H1N1 influenza virus. Nature 2009, 459: 931-939. 10.1038/nature08157PubMed CentralView ArticlePubMedGoogle Scholar
- Olson DR, Simonsen L, Edelson PJ, Morse SS: Epidemiological evidence of an early wave of the 1918 influenza pandemic in New York City. Proc Natl Acad Sci USA 2005, 102: 11059-11063. 10.1073/pnas.0408290102PubMed CentralView ArticlePubMedGoogle Scholar
- Andreasen V, Viboud C, Simonsen L: Epidemiologic characterization of the 1918 influenza pandemic summer wave in Copenhagen: implications for pandemic control strategies. J Infec Dis 2008, 197: 270-278. 10.1086/524065View ArticleGoogle Scholar
- Anders EM, Hartley CA, Jackson DC: Bovine and mouse serum β inhibitors of influenza A viruses are mannose-binding lectins. Proc Natl Acad Sci USA 1990, 87: 4485-4489. 10.1073/pnas.87.12.4485PubMed CentralView ArticlePubMedGoogle Scholar
- Hartley CA, Jackson DC, Anders EM: Two distinct serum mannose-binding lectins function as β inhibitors of influenza virus: identification of bovine serum β inhibitor as conglutinin. J Virol 1992, 66: 4358-4363.PubMed CentralPubMedGoogle Scholar
- Hartshorn KL, Sastry K, White MR, Anders EM, Super M, Ezekowitz RA, Tauber AI: Human mannose-binding protein functions as an opsonin for influenza A viruses. J Clin Invest 1993, 91: 1414-1420. 10.1172/JCI116345PubMed CentralView ArticlePubMedGoogle Scholar
- Anders EM, Hartley CA, Reading PC, Ezekowitz RA: Complement-dependent neutralization of influenza virus by a serum mannose-binding lectin. J Gen Virol 1994, 75: 615-622. 10.1099/0022-1317-75-3-615View ArticlePubMedGoogle Scholar
- Reading PC, Morey LS, Crouch EC, Anders EM: Collectin-mediated antiviral host defense of the lung: Evidence from influenza virus infection of mice. J Virol 1997, 71: 8204-8212.PubMed CentralPubMedGoogle Scholar
- Kase T, Suzuki Y, Kawai T, Sakamoto T, Ohtani K, Eda S, Maeda A, Okuno Y, Kurimura T, Wakamiya N: Human mannan-binding lectin inhibits the infection of influenza A virus without complement. Immunology 1999, 97: 389-392. 10.1046/j.1365-2567.1999.00781.xView ArticleGoogle Scholar
- Kawasaki T: Structure and biology of mannan-binding protein, MBP, an important component of innate immunity. Biochim Biophys Acta 1999, 1473: 186-195.View ArticlePubMedGoogle Scholar
- Fujita T, Matsushita M, Endo Y: The lectin-complement pathway-its role in innate immunity and evolution. Immunol Rev 2004, 198: 185-202. 10.1111/j.0105-2896.2004.0123.xView ArticlePubMedGoogle Scholar
- Summerfield JA, Taylor ME: Mannose-binding proteins in human serum: identification of mannose-specific immunoglobulins and a calcium-dependent lectin, of broader carbohydrate specificity, secreted by hepatocytes. Biochem Biophys Acta 1986, 883: 197-206.View ArticlePubMedGoogle Scholar
- Kawasaki N, Kawasaki T, Yamashina I: A serum lectin (Mannan-binding protein) has complement-dependent bactericidal activity. J Biochem 1989, 106: 483-489.PubMedGoogle Scholar
- Wilson IA, Skehel JJ, Wiley DC: Structure of the haemagglutinin membrane glycoprotein of influenza virus at 3 Å resolution. Nature 1981, 289: 366-373. 10.1038/289366a0View ArticlePubMedGoogle Scholar
- Influenza virus resource[http://www.ncbi.nlm.nih.gov/genomes/FLU/FLU.html]
- Ohuchi M, Ohuchi R, Sakai T, Matsumoto A: Tight binding of influenza virus hemagglutinin to receptor interferes with fusion pore dilation. J Virol 2002, 76: 12405-12413. 10.1128/JVI.76.24.12405-12413.2002PubMed CentralView ArticlePubMedGoogle Scholar
- Reid AH, Fanning TG, Hultin JV, Taubenberger JK: Origin and evolution of the 1918 "Spanish" influenza virus hemagglutinin gene. Proc Natl Acad Sci USA 1999, 96: 1651-1656. 10.1073/pnas.96.4.1651PubMed CentralView ArticlePubMedGoogle Scholar
- Kobasa D, Takada A, Shinya K, Hatta M, Halfmann P, Theriault S, Suzuki H, Nishimura H, Mitamura K, Sugaya N, Usui T, Murata T, Maeda Y, Watanabe S, Suresh M, Suzuki T, Suzuki Y, Feldmann H, Kawaoka Y: Enhanced virulence of influenza A viruses with the haemagglutinin of the 1918 pandemic virus. Nature 2004, 431: 703-707. 10.1038/nature02951View ArticlePubMedGoogle Scholar
- Accession number ADA79597, ADB81459, ADF42661, ADI99498[http://www.ncbi.nlm.nih.gov/genomes/FLU/FLU.html]
- Accession number CY053736, CY054678, HM123483, HM569740[http://www.ncbi.nlm.nih.gov/genomes/FLU/FLU.html]
- Hartshorn KL, White MR, Shepherd V, Reid K, Jensenius JC, Crouch EC: Mechanisms of anti-influenza activity of surfactant proteins A and D: comparison with serum collectins. Am J Physiol 1997, 273: 1156-1166.Google Scholar
- Hawgood S, Brown C, Edmondson J, Stumbaugh A, Allen L, Goerke J, Clark H, Poulain F: Pulmonary collectins modulate strain-specific influenza a virus infection and host responses. J Virol 2004, 78: 8565-8572. 10.1128/JVI.78.16.8565-8572.2004PubMed CentralView ArticlePubMedGoogle Scholar
- White MR, Crouch E, Vesona J, Tacken PJ, Batenburg JJ, Leth-Larsen , Holmskov U, Hartshorn KL: Respiratory innate immune proteins differentially modulate the neutrophil respiratory burst response to influenza A virus. Am J Physiol Lung Cell Mol Physiol 2005, 289: 606-616. 10.1152/ajplung.00130.2005View ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.