- Open Access
Complete genome sequence of the lytic Pseudomonas fluorescens phage ϕIBB-PF7A
© Sillankorva et al; licensee BioMed Central Ltd. 2011
- Received: 26 July 2010
- Accepted: 26 March 2011
- Published: 26 March 2011
Phage ϕIBB-PF7A is a T7-like bacteriophage capable of infecting several Pseudomonas fluorescens dairy isolates and is extremely efficient in lysing this bacterium even when growing in biofilms attached to surfaces. This work describes the complete genome sequence of this phage.
The genome consists of a linear double-stranded DNA of 40,973 bp, with 985 bp long direct terminal repeats and a GC content of approximately 56%. There are 52 open reading frames which occupy 94.6% of the genome ranging from 137 to 3995 nucleotides. Twenty eight (46.7%) of the proteins encoded by this virus exhibit sequence similarity to coliphage T7 proteins while 34 (81.0%) are similar to proteins of Pseudomonas phage gh-1.
That this phage is closely related to Pseudomonas putida phage gh-1 and coliphage T7 places it in the "T7-like viruses" genus of the subfamily Autographivirinae within the family Podoviridae. Compared to the genome of gh-1, the sequence of ϕIBB-PF7A is longer and contains more genes with unassigned function and lacks a few potentially essential and non-essential T7 genes, such as gene1.1, 3.8, and 7.
- Lysis Gene
- Nonessential Protein
- Direct Terminal Repeat
- Internal Virion Protein
- Display Sequence Similarity
Formerly, the phylogenetic and taxonomic classification of bacteriophages (phages) was based on similarities in phage morphology, host range , ability to recombine, and also on similar cross-hybridization patterns of their DNAs [2–4]. Today, phage genome sequencing is commonly performed for classification and characterization purposes and is based on the arrangement of conserved genes and the nucleotide and protein sequence identity [5, 6]. A specific group of phages has a number of conserved proteins but also a relatively high number of proteins for which no match occurs in the current databases. This reveals the great genetic diversity existent within phages. Of the total global estimated phage population (> 1031), only a small number have been completely sequenced.
In the last few years, a large number of T7-like phages have been sequenced most of which have been gathered in a new viral subfamily within the family Podoviridae. The Autographivirinae was formerly referred to as the T7 supergroup of phages . All these phages, most of which have members of the Enterobacteriaceae as their host, present a highly conserved genome organization and mainly differ at sequence level through the presence or absence of nonessential genes . Phages presenting more similarities to each other are grouped in genera within the Autographivirinae, namely the "T7-like viruses", the "SP6-like viruses" and the "phiKMV-like viruses". The genome of coliphage T7 has 56 genes encoding potential proteins , of which 35 have known function or functions. In addition, there are 25 nonessential proteins, of which only 12 are conserved across the genus "T7-like viruses" .
In this manuscript we report on the full genomic characterization of Pseudomonas fluorescens phage ϕIBB-PF7A, a phage capable of infecting several dairy P. fluorescens isolates including isolates belonging to different ribotypes . On the basis of TEM micrographs this virus resembled T7-like phages with a head diameter of about 63 nm and a noncontractile tail size approximately 13 × 8 nm. In terms of growth cycle, phage ϕIBB-PF7A has a latent period of 15 minutes, an eclipse period of 10 minutes and a burst size of 153 plaque forming units per infected cell. Furthermore, this phage has been the focus of several recent studies lysing efficiently its host present in single  and dual species biofilms . Moreover, ϕIBB-PF7A is the first reported phage capable of efficiently infecting elongated P. fluorescens cells - which are up to 10 times longer than normal sized cells - and killing planktonic stationary (3 days old) phase cells . For these reasons, a more detailed characterization was desired in order to further increase our knowledge about this particular lytic phage.
This work focuses on the determination of the complete genome sequence of the P. fluorescens phage ϕIBB-PF7A. This virus is the first T7-like bacteriophage for P. fluorescens for which the genome has been determined.
ϕIBB-PF7A nucleotide sequence and general sequence properties
The DNA sequence of phage ϕIBB-PF7A was determined and it consists of linear double-stranded DNA of 40,973 bp. The size of this phage correlates well with other T7-like phage members, where the smallest and largest phages reported so far are the Pseudomonas putida phage gh-1 (37.4 kb) and the Vibrio parahaemolyticus phage VpV262 (45.9 kb), respectively . The redundant terminal repeats (DTR) of ϕIBB-PF7A are unusually long (985 bp) for member of the T7 group (T7 has DTRs of only 160 bp ) and, up till now, the genome of P. aeruginosa phage LKD16 had the longest DTRs (428 bp) . Phage ϕIBB-PF7A has an overall genomic guanine plus cytosine (GC) content of 56.3%, well within the range of GC contents observed in other T7-like phages (46.2 - 62.3%) .
The genome of ϕIBB-PF7A was scanned for open reading frames (ORFs) of 100 bp or longer using computational software complemented with visual inspection of the genome sequence. The search resulted in 52 predicted genes with lengths ranging from 137 to 3995 nt. The initiation codon of 92% of the protein-coding genes is ATG and only four other ORFs, including RNA polymerase (gene1) and the packaging protein B (gene19), start with GTG. The temporal and functional distributions of genes are tightly organized and packed close to each other so that they occupy 94.6% of the entire nucleotide sequence.
Comparative genomics of phage ϕIBB-PF7A
All predicted protein-coding genes were screening using BLASTP against the non-redundant protein database at NCBI. From the 52 ORFs of ϕIBB-PF7A: i) 25 (48.1%) have assigned function; ii) 12 (23.1%) are similar to proteins of unknown function in the nonredundant databases and (iii) 15 (28.8%) code for hypothetical proteins unique to this bacteriophage. While the great majority (34) of the homologs is to proteins of Pseudomonas phage gh-1, examples of primary sequence similarity to Pseudomonas phages phi-2 and LKA1, coliphage T3, Yersinia phage φYeO3-12, Vibrio phage ICP3_2008_A and Kluyvera phage Kvp1 exist. With the exceptions of Pseudomonas phage LKA1 which is a member of the "phiKMV-like viruses," all of these phages are members of the "T7-like viruses" genus. Since 28 gene products were related to T7 phage proteins, the T7 gene nomenclature was adopted for these genes (Additional file 1, Table S1). No function can be speculated about the hypothetical proteins of phage ϕIBB-PF7A without further study. Based upon overall protein homology determined using CoreGenes [16, 17] this virus shares 28 (47%), 11 (21%) and 10 (20%), similar proteins with phages T7, SP6 and φKMV, respectively. These results indicate that it is a member of the Autographivirinae, specifically a member of the "T7-like viruses" genus .
Despite the different host ranges of P. fluorescens phage ϕIBB-PF7A and P. putida phage gh-1, there are 34 phage proteins which show significant sequence identity (Figure 1). As pointed out above, phage gh-1 belongs to the same viral genus as ϕIBB-PF7A and its sequence is linear double-stranded DNA of 37,359 bp with 216 bp direct terminal repeats, and has 43 predicted putative genes.
A Mauve alignment [16, 18] of phage ϕIBB-PF7A DNA against the genomes of gh-1, T3 and T7 show the regions of DNA sequence similarity (colored areas) interspersed with regions (white) where no sequence similarity exists (Additional file 2; Figure S1).
The genes essential of forming the channel which allows the phage DNA injection into the hosts' cytoplasm are all present in phage ϕIBB-PF7A, namely gene14, gene15 and gene16.
ϕIBB-PF7A gene arrangement
A. Early gene expression
The early genes of this type of virus are transcribed by the host RNA polymerase. The transcribed genes include genes which function to overcome host restriction and to convert the metabolism of the host cell to the production of phage proteins, e.g. synthesis of the phage promoter-specific RNA polymerase (RNAP; gene1). Two host-type promoters were discovered (Additional file 3; Table S2). Interestingly, the early region of this phage contains six ORFs, two of which are in the LTR, which show no sequence similarity at the DNA or protein level to the early genes of any other member of the Autographivirinae. Indeed, this is the first report for the existence of putative ORFs within the terminal repeats of a member of this subfamily, though they are found in phages such as T5 and SPO1. ϕIBB-PF7A does not encode the T7-like Gp0.3 (ocr), 0.6 or 0.7 homologs.
B. Middle gene expression
The class II genes are transcribed by the phage RNAP and are involved in DNA replication. Both phages ϕIBB-PF7A and gh-1 lack several class II genes present in T7, namely: gene1.4, gene1.5, gene1.6, gene1.7, gene1.8, gene3.2, gene3.7, gene4.3, and gene5.5. Most of these class II genes are nonessential and only gene1.7 is considered an essential gene and reportedly is beneficial for phage growth . Besides the genes not present in both ϕIBB-PF7A and gh-1, ϕIBB-PF7A is also missing the nonessential homing endonuclease (gene3.8).
C. Late gene expression
The late genes or class III genes are transcribed by the phage RNAP and are mostly involved in morphogenesis and host lysis. The majority of putative genes found in T7-like phages are also present in ϕIBB-PF7A (Additional file 1, Table S1). Phage ϕIBB-PF7A is only missing gene7, a nonessential protein which is usually involved in host range . Compared to gh-1, phage ϕIBB-PF7A has more ORFs situated just before the right DTR (Figure 1). We have identified two ORFs which display sequence similarity to the major capsid proteins of phages belonging to the "T7-like viruses." The upstream gene displays homology to the capsid gene of Yersinia phage φYeO3-12 while the downstream gene is similar to the analogous protein in P. putida phage gh-1. That these two genes might be coexpressed as a single fusion protein is suggested by the presence of a slippery site (GTTATCGAAAAGGCGTAA) which is translated as VIEKA(stop); but could be extended, via -1 frameshifting, into VIEKgvsvpdp...(stop). While a diagnostic pseudoknot was discovered immediately downstream of the potential slippery site [19–22] there is no proteomic evidence for frameshifting in this bacteriophage . The structural proteome of this phage is therefore made up of gene10A (capsid), the connector gene8, and a conspicuous core made by the proteins gene14, gene15, and gene16 .
Phage ϕIBB-PF7A contains gene16, the internal virion protein D, which contains peptidoglycan hydrolase motif. This activity is essential for enlarging a hole across the cell wall of cells when hosts are infected at low temperatures . Possibly, due to the presence of this protein phage ϕIBB-PF7A showed a high efficiency towards mature biofilms .
Endolysins are muralytic enzymes produced by dsDNA phages which hydrolyze the peptidoglycan layer of bacterial cell walls. As in T7 phages, gene3.5 of ϕIBB-PF7A is proposed to be the endolysin-like protein which possesses N-acetylmuramoyl-L-alanine amidase activity, but is apparently not essential for lysis (I. Molineux, personal communication). The access of endolysins to the cell wall occurs through the presence of a secondary lysis factor known as holin. The small membrane protein derived from gene17.5 of ϕIBB-PF7A is proposed as holin and it showed highest sequence similarity to Morganella phage MmP1 lysis protein. Phage ϕIBB-PF7A has also one more lysis gene, the λ Rz-like lysis protein (gene18.5) which presents a 34% sequence identity to the homologous protein in phage gh-1. The proteins of phage ϕIBB-PF7A was scanned for transmembrane helices using TMHMM. A total of six ORFS contain transmembrane helices (TMD, orf2, gene17.5, gene18.5, gene19, and gene19.5, and orf52), with only one (gene17.5) presenting two TMD.
Putative regulatory elements
No tRNA genes were predicted in the ϕIBB-PF7A genome using tRNAscan-SE. Several potential putative promoters were identified in the DNA sequence of ϕIBB-PF7A, using PHIRE and BPROM (Additional file 3, Table S2). Using strict criteria for the assignment of host promoter sites, i.e. that four out of the six bp in the -10 and -35 regions be conserved, two host-dependent promoters were discovered. These are both located in orf4. We have been unable to definitively assign promoters to earlier ORFs.
The promoter sequences all lie in intergenic regions and show the greatest sequence similarity to those of coliphage T3 (Additional file 3, Table S2). The specificity of the RNAP is determined by the positions -10 and -11 and it is the position -2 that establishes the promoter strength . The highest relative utilization of the promoter is observed when there is a T at the -2 position. Phage ϕIBB-PF7A may have lower promoter utilization since there is not a T positioned at -2. Nonetheless, the promoter positions -10 and -11 are identical to the consensus sequence of T3 and SP6 (C s, respectively). In phage ϕIBB-PF7A, seven of the promoters were found to be present in identical places as in phage T7: ϕ1, ϕ2.5, ϕ6.5, ϕ9, ϕ10A, ϕ17and ϕ19.5, respectively. The promoter sequences which were found in analogous genomic positions to the ones in T7 suggest that the regulation of phage mRNA synthesis is well conserved within the group.
FindTerm, RibEx and TransTerm programs were used to search for transcriptional terminators. T7 and gh-1 phages have three terminators identified while phage ϕIBB-PF7A has only one rho-independent terminator downstream of gene10 (22995 to 23040) which suggest the presence of the Tø2 terminators (named TLate1 in phage gh-1). Downstream of gene1.3 is a sequence (gccctgtaccttgcagggc) which exhibits a ΔG of -11.60 kcal/mol. While this sequence is identically placed to the rho-independent terminator of most T7-like phages it lacks the classical polyT tail.
Summarizing, the genome sequencing of ϕIBB-PF7A showed similarity to "T7-like viruses" of the subfamily Autographivirinae and allowed the recognition of a new subgroup consisting, so far, of only phages ϕIBB-PF7A and gh-1.
Bacteria and bacteriophage ϕIBB-PF7A
The bacterium used (P. fluorescens PF7) was isolated from a dairy industry (Estação Experimental, Paços de Ferreira, Portugal), identified using the API 20NE panel (BioMérieux S.A., France), and grown at 30°C in Tryptic Soy Broth (TSB, Fluka) or TSB agar plates for bacteriophage propagation. Bacteriophage ϕIBB-PF7A was isolated from raw sewage and has been previously characterized according to morphology, growth cycle and other parameters .
Bacteriophage ϕIBB-PF7A propagation
For phage amplification, the plate lysis and elution method described by Sambrook & Russell  was used with some modifications. Briefly, 1 ml of phage (1 × 103 PFU ml-1) and 1 ml of overnight grown host were mixed with 30 ml of TSB soft agar (TSB + 0.6% agar) and added to 250 ml T-flasks with a thin layer of TSA media. The soft-agar layer was allowed to solidify and the flasks were incubated at 30°C for 7 h. The phage particles were subsequently eluted with SM buffer and concentrated with PEG 8000, and then extracted with chloroform. Samples in SM buffer were stored at 4°C until further use.
Phage DNA was isolated using a Wizard Lambda Preps DNA purification system (Promega, Madison, WI.) according to the manufacturer's instructions.
Phage DNA sequencing
Phage ϕIBB-PF7A DNA was sheared with a nebulizer. Following 1% agarose gel electrophoresis overnight electrophoresis at 30 V, fractions with sizes between 1 and 4 kb were cut from the gels and the DNA purified. The ends were repaired, the fragments were ligated overnight, at 16°C, into pZero cloning vector (Zero background/Kan cloning kit, Invitrogen, Carlsbad, CA) previously digested with EcoRV, and transformed into chemically competent One Shot® TOP10 cells (Invitrogen). The cells were recovered in SOC medium, incubated for 1 h at 37°C and 225 rpm (Titramax plate shaker, Heidolph Elektro GmbH & Co. KG, Kelheim, Germany) and plated onto LB-Kan plates containing X-Gal. Selected clones were picked with a Genetix QPix2 robotic colony picker (Genetix Corp., Boston, MA) and grown overnight in LB medium at 37°C and 225 rpm. The cultures were centrifuged and plasmid DNA was extracted. For sequencing, Big Dye chemistry was used and the sequencing was carried out in ABI 3700 DNA Sequencer (Applied Biosystems). Sequence reads were assembled using GAP4 (Staden package software) and the sequences were assembled into contigs .
Several frameshift errors were corrected, through a primer-walking technique with 20-mer oligonucleotide primers and pure ϕIBB-PF7A DNA with the amplicons being sequenced.
ORF prediction and annotation
Prediction of open reading frames (ORFs) was performed using GeneMark.hmm, OrfFinder and Kodon (Applied Maths, Austin, TX). For tRNA gene identification, the tRNAscan-SE program was used .
Translated ORFs were compared with known protein sequences using BLASTP, against the non-redundant protein GenBank database. To tease out function conserved hypothetical proteins were also analyzed by HHPred at http://toolkit.tuebingen.mpg.de/hhpred.
Molecular masses and isoelectric points of phage ϕIBB-PF7A translated putative proteins were determined using the ExPASy Compute pI/Mw tool http://au.expasy.org/tools/pi_tool.html. Promoter predictions were made using promoter predictor http://www.fruitfly.org/seq_tools/promoter.html, PHIRE 1.0  and BPROM http://www.fruitfly.org/seq_tools/promoter.html and the search for transmembrane helices was done by using TMHMM http://www.cbs.dtu.dk/services/TMHMM-2.0/. Terminators were predicted using FindTerm http://www.cbs.dtu.dk/services/TMHMM-2.0, RibEx http://220.127.116.11:8080/cgi-bin/ribex.cgi and TransTerm http://nbc11.biologie.uni-kl.de/framed/left/menu/auto/right/transterm/.
Nucleotide sequence accession number
The GenBank accession number of the complete genomic sequence of phage ϕIBB-PF7A is GU583987.
SS acknowledges the financial support from the Portuguese Foundation for Science and Technology (FCT) (grant SFRH/BD/18485/2004). AK acknowledges the financial support of a discovery grant from the Natural Sciences and Engineering Research Council of Canada.
- Adams MH: Bacteriophages. New York: Interscience Publishers; 1959.Google Scholar
- Botstein D: A theory of modular evolution for bacteriophages. Ann N Y Acad Sci 1980, 354: 484-490. 10.1111/j.1749-6632.1980.tb27987.xView ArticlePubMedGoogle Scholar
- Campbell A: Comparative molecular biology of lambdoid phages. Annu Rev Microbiol 1994, 48: 193-222. 10.1146/annurev.mi.48.100194.001205View ArticlePubMedGoogle Scholar
- Casjens S, Wyckoff E, Hayden M: Bacteriophage P22 portal protein is part of the gauge that regulates packing density of intravirion DNA. Journal of Molecular Biology 1992, 224: 1055-1074. 10.1016/0022-2836(92)90469-ZView ArticlePubMedGoogle Scholar
- Brüssow H, Desiere F: Comparative phage genomics and the evolution of Siphoviridae : insights from dairy phages. Mol Microbiol 2001, 39: 213-222.View ArticlePubMedGoogle Scholar
- Lucchini S, Desiere F, Brüssow H: Comparative genomics of Streptococcus thermophilus phage species supports a modular evolution theory. J Virol 1999, 73: 8647-8656.PubMed CentralPubMedGoogle Scholar
- Lavigne R, Seto D, Mahadevan P, Ackermann HW, Kropinski AM: Unifying classical and molecular taxonomic classification: analysis of the Podoviridae using BLASTP-based tools. Research in Microbiology 2008, 159: 406-414. 10.1016/j.resmic.2008.03.005View ArticlePubMedGoogle Scholar
- Molineux IJ: The T7 Group. In The bacteriophages. Edited by: Calendar RL. Oxford: Oxford University Press; 2005.Google Scholar
- Sillankorva S, Neubauer P, Azeredo J: Isolation and characterization of a T7-like lytic phage for Pseudomonas fluorescens . Bmc Biotechnology 2008, 8: 80. 10.1186/1472-6750-8-80PubMed CentralView ArticlePubMedGoogle Scholar
- Sillankorva S, Neubauer P, Azeredo J: Pseudomonas fluorescens biofilms subjected to phage IBB-PF7A. Bmc Biotechnology 2008, 8: 79. 10.1186/1472-6750-8-79PubMed CentralView ArticlePubMedGoogle Scholar
- Sillankorva S, Neubauer P, Azeredo J: Phage control of dual species biofilms of Pseudomonas fluorescens and Staphylococcus lentus . Biofouling 2008.Google Scholar
- Kovalyova IV, Kropinski AM: The complete genomic sequence of lytic bacteriophage gh-1 infecting Pseudomonas putida --evidence for close relationship to the T7 group. Virology 2003, 311: 305-315. 10.1016/S0042-6822(03)00124-7View ArticlePubMedGoogle Scholar
- Dunn JJ, Studier FW: Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements. Journal of Molecular Biology 1983, 166: 477-535. 10.1016/S0022-2836(83)80282-4View ArticlePubMedGoogle Scholar
- Ceyssens P-J, Lavigne R, Mattheus W, Chibeu A, Hertveldt K, Mast J, Robben J, Volckaert G: Genomic Analysis of Pseudomonas aeruginosa Phages LKD16 and LKA1: Establishment of the KMV Subgroup within the T7 Supergroup. Journal of Bacteriology 2008, 188: 6924-6931. 10.1128/JB.00831-06View ArticleGoogle Scholar
- Lavigne R, Burkal'tseva MV, Robben J, Sykilinda NN, Kurochkina LP, Grymonprez B, Jonckx B, Krylov VN, Mesyanzhinov VV, Volckaert G: The genome of bacteriophage ϕKMV, a T7-like virus infecting Pseudomonas aeruginosa . Virology 2008, 312: 49-59. 10.1016/S0042-6822(03)00123-5View ArticleGoogle Scholar
- Kropinski AM, Borodovsky M, Carver TJ, Cerdeño-Tárraga AM, Darling A, Lomsadze A, Mahadevan P, Stothard P, Seto D, Van Domselaar G, Wishart DS: In silico identification of genes in bacteriophage DNA. Methods Mol Biol 2009, 502: 89.Google Scholar
- Zafar N, Mazumder R, Seto D: CoreGenes: a computational tool for identifying and cataloging "core" genes in a set of small genomes. BMC Bioinformatics 2002, 24: 3-12.Google Scholar
- Darling AC, Mau B, Blattner FR, Perna NT: Mauve: multiple alignment of conserved genomic sequence with rearrangements. Genome Res 2004, 14: 1394-1403. 10.1101/gr.2289704PubMed CentralView ArticlePubMedGoogle Scholar
- Alam SL, Atkins JF, Gesteland RF: Programmed ribosomal frameshifting: Much ado about knotting! Proceedings of the National Academy of Sciences of the United States of America 1999, 96: 14177-14179. 10.1073/pnas.96.25.14177PubMed CentralView ArticlePubMedGoogle Scholar
- Farabaugh PJ: Programmed translational frameshifting. Annual Review of Genetics 1996, 30: 507-528. 10.1146/annurev.genet.30.1.507View ArticlePubMedGoogle Scholar
- Harger JW, Meskauskas A, Dinman JD: An 'integrated model' of programmed ribosomal frameshifting. Trends in Biochemical Sciences 2002, 27: 448-454. 10.1016/S0968-0004(02)02149-7View ArticlePubMedGoogle Scholar
- Reeder J, Steffen P, Giegerich R: pknotsRG: RNA pseudoknot folding including near-optimal structures and sliding windows. Nucleic Acids Research 2007, 35: W320-W324. 10.1093/nar/gkm258PubMed CentralView ArticlePubMedGoogle Scholar
- Garcia LR, Molineux IJ: Transcription-independent DNA translocation of bacteriophage T7 DNA into Escherichia coli. Journal of Bacteriology 1996, 178: 6921-6929.PubMed CentralPubMedGoogle Scholar
- Klement JF, Moorefield MB, Jorgensen E, Brown JE, Risman S, McAllister WT: Discrimination between bacteriophage T3 and T7 promoters by the T3 and T7 RNA polymerases depends primarily upon a three base-pair region located 10 to 12 base-pairs upstream from the start site. Molecular Biology 1990, 215: 21-29. 10.1016/S0022-2836(05)80091-9View ArticleGoogle Scholar
- Sambrook J, Russell DW: Molecular Cloning: A Laboratory Manual. New York: Cold Spring Harbor Laboratory Press, Cold Spring Harbor; 2001.Google Scholar
- Staden R, Beal KF, Bonfield JK: The Staden package, 1998. Methods Mol Biol 2000, 132: 130.Google Scholar
- Lowe TM, Eddy SR: tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence. Nucleic Acids Res 1997,1(25):955-964. 10.1093/nar/25.5.955View ArticleGoogle Scholar
- Lavigne R, Sun WD, Volckaert G: PHIRE, a deterministic approach to reveal regulatory elements in bacteriophage genomes. Bioinformatics 2004, 20: 629-U99. 10.1093/bioinformatics/btg456View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.