Figure 5

APE1/Ref-1 increases transactivation by EBNA1, but does not alter transactivation by Tat. (A) C33a cells were co-transfected with reporter and effector plasmids, and the indicated amount of an APE1/Ref-1 expression plasmid. The backbone expression plasmid, pcDNA3.1 was used to normalize the amount of DNA used in each transfection. Reporter activity was measured 24 hours post-transfection. Transactivation is expressed as a percent of transactivation observed in the absence of co-transfected pAPE1 (control conditions). The inset legend indicates the columns corresponding to each effector/reporter combination. (B) Immunoblots indicate that expression of APE1/Ref-1 did not alter expression of EBNA1 or Tat. β-actin was used as a loading control. Asterisks indicate statistical significance by the Wilcoxon rank-sum test (p < 0.05) for APE1/Ref-1 transfected cells compared to control cells where pcDNA3.1 was co-transfected with the effector and reporter plasmids.