Table 2 Sequence of the primers used in real-time PCR
Genes | Direction | Sequence | Product (bp) | Accession no. in GenBank |
|---|---|---|---|---|
GAPDHa | Forward | TGCCATCACAGCCACACAGAAG | 123 | AF047874.1 |
| Â | Reverse | ACTTTCCCCACAGCCTTAGCAG | Â | Â |
IFN-γa | Forward | AAGTCAAAGCCGCACATCAAAC | 132 | X99774.1 |
| Â | Reverse | CTGGATTCTCAAGTCGTTCATCG | Â | Â |
IL-2 | Forward | TTCTGGGACCACTGTATGCTCTT | 129 | AF000631.1 |
| Â | Reverse | TACCGACAAAGTGAGAATCAATCAG | Â | Â |
IL-12P40 | Forward | CGAAGTGAAGGAGTTCCCAGAT | 123 | AY262752.1 |
| Â | Reverse | GACCGTATCATTTGCCCATTG | Â | Â |
IL-4 | Forward | AATGACATCCAGGGAGAGGTTTC | 100 | AJ621249.1 |
| Â | Reverse | AGGCTTTGCATAAGAGCTCAGTTT | Â | Â |
IL-5 | Forward | GGAACGGCACTGTTGAAAAATAA | 111 | AJ621252.1 |
| Â | Reverse | TTCTCCCTCTCCTGTCAGTTGTG | Â | Â |
IL-13 | Forward | CTGCCCTTGCTCTCCTCTGT | 123 | AJ621250.1 |
| Â | Reverse | CCTGCACTCCTCTGTTGAGCTT | Â | Â |
IL-10 | Forward | GCTGAGGGTGAAGTTTGAGGAA | 142 | AF000631.1 |
| Â | Reverse | GAAGCGCAGCATCTCTGACA | Â | Â |
H strain | Forward | CACTCCCTGGTGGCGTTTA | 126 | AY321955.1 |
| Â | Forward | TGTCGTTGATGTTGGCTGTTG | Â | Â |
Ts strain | Forward | ACCGGCACCGACAACCTTA | 117 | AF076230.1 |
| Â | Reverse | CCCTGCCTGACCACCACTT | Â | Â |