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Table 1 Standard curve data from real-time PCR

From: Comparison of the expression of cytokine genes in the bursal tissues of the chickens following challenge with infectious bursal disease viruses of varying virulence

Genes

Equation of standard curve

Correlation coefficient (r2)

GAPDH

Y = -3.48X + 39.02

0.997

IFN-γ

Y = -3.58X + 39.08

0.994

IL-2

Y = -3.62X + 37.54

0.994

IL-12P40

Y = -3.36X + 36.05

0.995

IL-4

Y = -3.16X + 39.42

0.991

IL-5

Y = -3.74X + 40.46

0.995

IL-13

Y = -4.02X + 40.87

0.991

IL-10

Y = -3.53X + 37.18

0.991

H strain

Ts strain

Y = -3.50X + 39.94

Y = -3.60X + 40.50

0.992

0.994

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Virology Journal

ISSN: 1743-422X