Serial bone marrow transplantation reveals in vivo expression of the pCLPG retroviral vector

Table 1 Summary of transplant procedure utilized

	1° Transplant	2° Transplant	3° Transplant
Survival of non-transplanted control animals	18-21d (n = 3)	18-22d (n = 3)	17-21d (n = 3)
Donor animals (male, C57BL/6)	n = 17	NA	NA
BMC collected	1-1.5 × 10⁷ total cells/donor	1-1.5 × 10⁷ total cells/donor	1-1.5 × 10⁷ total cells/donor
Total number of recpients (female, C57BL/6)	n = 18	n = 17	n = 12
Donor animals used for serial transplant	n = 6^a	n = 5^b	NA
Short term maintenance of transplant recipients	64 d (n = 6)	59 d (n = 6)	62 d (n = 6)
Long term maintenance of transplant recipients	10 m (n = 6)^c	8 m (n = 6)^c	6 m (n = 6)^c

a, Primary transplant recipients that were used as donors in the secondary transplant
b, Secondary transplant recipients that were used as donors in the tertiary transplant
c, Three animals in each long term group received 1 mg/kg 5-aza 24h prior to sacrifice

ISSN: 1743-422X