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Studies on membrane topology, N-glycosylation and functionality of SARS-CoV membrane protein
Virology Journalvolume 6, Article number: 79 (2009)
The glycosylated membrane protein M of the severe acute respiratory syndrome associated coronavirus (SARS-CoV) is the main structural component of the virion and mediates assembly and budding of viral particles. The membrane topology of SARS-CoV M and the functional significance of its N-glycosylation are not completely understood as is its interaction with the surface glycoprotein S. Using biochemical and immunofluorescence analyses we found that M consists of a short glycosylated N-terminal ectodomain, three transmembrane segments and a long, immunogenic C-terminal endodomain. Although the N-glycosylation site of M seems to be highly conserved between group 1 and 3 coronaviruses, studies using a recombinant SARS-CoV expressing a glycosylation-deficient M revealed that N-glycosylation of M neither influence the shape of the virions nor their infectivity in cell culture. Further functional analysis of truncated M proteins showed that the N-terminal 134 amino acids comprising the three transmembrane domains are sufficient to mediate accumulation of M in the Golgi complex and to enforce recruitment of the viral spike protein S to the sites of virus assembly and budding in the ERGIC.
Coronaviruses have a broad range of vertebrate hosts and usually cause mild respiratory diseases in humans and animals [1–3]. In March 2003 the world health organization issued a global alert about a severe partially fatal respiratory disease named severe acute respiratory syndrome (SARS). SARS originated in Southeast China, affected thousands and spread to many countries worldwide via international travel. Drastic quarantine measures and tight travel restrictions finally contained the SARS outbreak [4, 5]. In parallel, the etiologic agent of the outbreak was identified with unprecedented speed and turned out to be a novel coronavirus with several distinguishing features to known coronaviruses [6–8]. The SARS outbreak showed drastically that coronaviruses can develop into highly pathogenic agents with the potential to threaten public health and global economy severely [9, 10].
The coronaviral membrane protein (M) is the most abundant protein in the viral envelope and fulfils pivotal functions in the viral life cycle. Besides mediating incorporation of the nucleocapsid into the newly formed virions, M recruits all other viral structural components to the ER-Golgi-intermediate compartment (ERGIC) where virus assembly and budding takes place [11–13]. While the topology of SARS-CoV M is yet unknown and in silico analyses of its topology revealed partially contradictory results (Fig. 1), previous publications showed that other coronaviral M proteins consist of three transmembrane domains with either an N-terminal ecto- and a C-terminal endodomain (Nexo-Cendo) or an Nexo-Cexo orientation [14–16].
SARS-CoV M is N-glycosylated at asparagine residue at position 4 and accumulates at steady state in the Golgi complex . Whether glycosylation of M plays a role in its intracellular distribution and assembly of viral particles is not known. Immunofluorescence analyses demonstrated that M is mainly retained in the ERGIC [17, 18].
In the present study we have investigated the topology of M and found that M consists of an N-terminal ectodomain containing the single N-glycosylation site, three transmembrane domains and a long cytosolic C-terminus. N-glycosylation is no prerequisite for accumulation of M in perinuclear regions. By using an infectious clone of SARS-CoV with a substitution in the N-glycosylation site of M, we observed that N-glycosylation is dispensable for assembly and infectivity of the virus. Furthermore, the hydrophobic N-terminus of M containing the three transmembrane domains is sufficient to recruit the SARS-CoV spike protein S to the budding compartment suggesting protein-protein-interaction between M and S via the transmembrane domains of M.
Topology of SARS-CoV M
To predict membrane-spanning helices in SARS-CoV M, we performed hydrophobicity analyses and secondary structure predictions using several computer programs (Fig. 1A). Three in four algorithms predicted three transmembrane helices at similar positions. However, the tmap-program predicted only two hydrophobic domains between amino acids 73–93 and 133–150. Further in silico analyses also differed in the prediction of the topology of the N- and the C-terminus of M.
To analyze the membrane topology of M experimentally, we benefited from the fact that a fraction of recombinant FLAG-tagged M is detectable at the plasma membrane . The FLAG epitope was fused to the N-terminus of M and the glycosylation-deficient mutant MN4Q and the accessibility of the FLAG Tag at the cell surface was analyzed by immunofluorescence of native cells expressing the two constructs. The experiments revealed that MFLAG as well as MN4Q-FLAG were detectable at the plasma membrane (Fig 1B). These results suggested that both M and MN4Q exhibit a Nexo-orientation with the N-terminus facing the luminal or extracellular space (Fig. 1B, right graphic).
To further characterize the membrane integration of M, we introduced a novel glycosylation site (N-X-S) in the predicted intraluminal loop between hydrophobic domains 2 and 3 of the protein (Fig. 1C). To avoid a very close proximity of the glycosylation site and the ER membrane leading to sterical limitations of the oligosaccharide transferase in the ER lumen , we inserted 31 hydrophilic amino acids with a central N-glycosylation motif between amino acids 73 and 74 of M and MN4Q (named MInsert; MN4Q Insert). The in vitro expression of MInsert resulted in three protein bands (Fig. 1C, lanes 1 and 4) which could be attributed to non-, mono- and di-glycosylated forms of M by Endo H digest (Fig. 1C, lane 5). Expression of MN4Q Insert which contains only the novel glycosylation site between the potential transmembrane segments 2 and 3 resulted in two M-specific bands (Fig. 1C, lanes 2 and 6). Endo H digestion revealed that the upper band was glycosylated suggesting that the two forms correspond to non- and monoglycosylated MN4Q Insert. (Fig. 1C, lane 7). These results demonstrate that the linker-domain between the putative second and third hydrophobic domains faces the luminal site and the two hydrophobic stretches are probably incorporated in the lipid-layer. Therefore we hypothesize a membrane topology of M as depicted in the right graphic in Fig. 1C.
In silico analysis using the computer program tmap identified a hydrophobic stretch between amino acids 133 and 150 suggesting an additional transmembrane domain, which would direct the C-terminus of M into the lumen. (Fig. 1A). To analyse, whether parts of the C-terminus of SARS-CoV M are located in the cytoplasm, we constructed further glycosylation mutants of M carrying single N-glycosylation motifs at position 186 (MV186N) or 205 (ML205N) (Fig. 1D). In vitro translation of these constructs in the presence of microsomes revealed that neither MV186N nor ML205N were glycosylated (Fig. 1D, lanes 2 and 3) suggesting that the C-terminus of M is completely located in the cytoplasm (Fig. 1D, right graphic).
In summary, we could for the first time provide experimental support for a Nexo-Cendo topology of SARS-CoV M with a luminal N-terminus, three transmembrane segments and a long cytosolic C-terminus.
Suppressed N-glycosylation of M does not impair protein accumulation in the Golgi complex nor the assembly and infectivity of a recombinant SARS-CoV
Amino acid sequence alignments demonstrated that the N-glycosylation motif at the extreme N-terminus of M is conserved between coronaviruses of group 1 and 3 and the newly emerged SARS-CoV (Fig. 2A). To confirm these in silico data the N-terminal N-glycosylation site of the homologue protein of another human pathogenic coronavirus, HCoV-NL63, was mutated which led to the loss of glycosylation completely as it is the case for SARS-CoV M (data not shown) . These results induced the idea that N-glycosylation might be important for protein transport, protein-protein-interactions or other steps in the viral life cycle. Therefore, the intracellular distribution of the glycosylation-deficient mutant MN4Q of SARS-CoV was investigated by immunofluorescence analysis (Fig. 2B). The staining of the ER-Golgi intermediate compartment (ERGIC) and the recombinantly expressed MN4Q revealed a strong colocalization suggesting that the MN4Q as well as wild type M accumulates in the budding compartment ERGIC and that N-glycosylation has no influence on intracellular transport of M (Fig. 2B, right image).
Recently, a T7 promoter-driven infectious cDNA-clone of SARS-CoV was engineered . To study N-glycosylation of M in the viral context, we applied this reverse genetic system and constructed a recombinant SARS-CoV (recSARS-CoV) expressing a glycosylation-deficient M. Expression of viral proteins was confirmed by western blot analysis using serum of a SARS-convalescent or the human monoclonal α-M antibody S30 . The serum recognized the structural components spike- (S) and nucleocapsid- (N) protein in cell lysates of wt and mutant recSARS-CoV infected Vero-cells. The M specific antibody could indeed detect the unglycosylated and the N-glycosylated form of M in wt recSARS-CoV infected Vero-cells (Fig. 3A). However, in cells infected with MN4Q recSARS-CoV only the unglycosylated form of M was detected. These results provide evidence that we successfully rescued a replication-competent recSARS-CoV expressing a glycosylation-deficient M. To illustrate the morphology of wt as well as mutant recSARS-CoV, we performed electronmicroscopy analysis of purified virions demonstrating that both virions share a characteristic corona-solis like appearance (Fig. 3B, upper panel). By measuring several virions we observed that the average size of wt and mutant virions was the same.
To compare growth properties between wt recSARS-CoV and mutant recSARS-CoV, we first used plaque assays. As shown in Fig. 3B, the plaque size of the mutant virus was similar to wt virus presuming that the spread of the virus expressing a glycosylation-deficient M is not inhibited (Fig. 3B, lower panel). To further analyze the growth kinetics of both viruses, cell cultures were infected and the viral replication efficiency was assessed by calculating the number of viral genomes in the supernatant by real-time RT-PCR (Fig. 3C). In FRHK4 cells (fetal rhesus monkey kidney), wt recSARS-CoV (blue bars) as well as MN4Q recSARS-CoV (red bars) exhibited a considerable 106-fold increase in viral genomes copies within 18 h p.i. and reached a plateau of approximately 107 viral genomes late in infection (40 h p.i.; Fig. 3C, left panel). These results were independent of the used cells since infection of Vero cells with both viruses resulted in similar amounts of viral particles late in infection (40 h p.i.; Fig. 3C, right panel). However, infection with MN4Q recSARS-CoV resulted in a 10-fold reduction of released genome copies early in infection compared to wt rec SARS-CoV. In general, both recombinant viruses possessed a delayed viral replication in infected Vero cells compared to FRHK4 cells.
In summary, we could provide evidence that a suppressed N-glycosylation of SARS-CoV M do not have a significant impact on protein transport and on assembly and replication of new virions in cell culture.
The hydrophilic cytosolic tail of M exhibits an immunogenic epitope
Previously, we could identify the M-specific antibody S30 synthesized by Epstein Barr virus-immortalized B-lymphocytes of a SARS convalescent . To map the epitope of this unique M-specific human monoclonal antibody, we constructed FLAG-tagged mutants with truncations in the C-terminal part of M. The respective mutants were expressed in BHK-T7 cells and employed in immunoblot and immunoprecipitation analysis (Fig. 4A and 4B). M and M1–134 were metabolically labelled during in vitro translation and precipitated using S30. While M was readily precipitated by S30, M1–134 was not (Fig. 4B). To narrow down the epitope recognized by S30 in the C-terminus of M, FLAG-tagged mutants of M were constructed which carried small truncations in the absolute C-terminus (Fig. 4C and 4D). Mutants were expressed in BHK-T7 cells and cell lysates were subjected to western blot analysis. While all mutants were readily detected by an anti-FLAG antibody, only mutants consisting of at least 201 amino acids could be detected by S30 (Fig. 4D, lanes 5 and 6). Unexpectedly, mutant M1–196 comprising of 196 amino acids migrated slightly slower than the mutant consisting of 201 amino acids. Since the sequence of the M1–196 had been verified like all other constructs, the reason for the abnormal behaviour in SDS-PAGE remained unknown.
We were not able to detect the truncated mutant M1–189. Transient expression of MN/C1-189 possessing a FLAG-tag at the N- and the C-terminus and subsequent Western blot analysis revealed the expression of the mutant, which was not detected by the S30 antibody. Taken together, it appears that S30 recognizes a distinct linear epitope located at the extreme C-terminus of M around amino acid residues 196 and 201.
The hydrophobic N-terminus of M is sufficient to recruit M in the Golgi-complex and to retain S in perinuclear regions
M is concentrated in the Golgi compartment but the molecular determinants for the intracellular distribution are not understood. Therefore, we analyzed the intracellular distribution of the C-terminal truncated mutant M1–134 by immunofluorescence (Fig. 5). As expected, full-length M accumulated in Golgi complex of transiently transfected Huh7-cells (Fig. 5, upper row). The recombinant expression of M1–134 showed strong colocalization with Golgi-marker as well suggesting that the hydrophobic N-terminus of M constitutes a Golgi-retention signal (Fig. 5, lower row).
The recruitment of all coronaviral structural components in the perinuclear region is crucial for viral assembly and budding. However, recombinant spike protein S was transported to the plasma membrane upon single expression (Fig. 6A, left panel), while S in SARS-CoV infected cells was localized mainly to the perinuclear region (Fig. 6A, right panel). Thus, we explored whether M influenced intracellular localization of S. To this end, M and S were coexpressed and their respective intracellular localization was analyzed by immunofluorescence (Fig. 6B, upper row). We found that in the presence of M, S was recruited to cellular compartments where SARS-CoV budding takes place. Replacing M by the mutant containing only the hydrophobic N-terminal part of the protein, M1–134, we observed that S is still found in the perinuclear region and plasma membrane transport is prevented (Fig. 6B, lower row). In summary this experiment showed that coexpression with at least the first 134 amino acids of M induced a redistribution of the surface protein S to the perinuclear region which is consistent with the idea that M recruited S to site of assembly and budding of SARS-CoV.
M proteins of coronaviruses with low pathogenic potential for humans are quite well understood in terms of synthesis, transport and function. M protein of the highly pathogenic SARS-CoV shows little amino acid sequence homology to other coronaviruses which makes predictions difficult . In this report, we analyzed SARS-CoV M with respect to membrane topology, N-glycosylation and functional interaction with the surface protein S.
Membrane topology and functional analysis of M
As shown previously, SARS-CoV M acquires one oligosaccharide side chain at asparagine residue 4 suggesting a luminal orientation of the N-terminus . This result was supported and extended in the present manuscript by immunofluorescence analyses demonstrating that even the glycosylation-deficient M exhibits an N-terminal ectodomain. The topology of the hydrophobic stretches in M was determined using artificially incorporated N-glycosylation sites postulating the presence of three transmembrane domains and a long cytosolic C-terminus. The topology is different to other coronaviruses e.g. the transmissible gastroenteritis virus (TGEV) M . The presented membrane topology model of SARS-CoV M presumes a "Nexo-Cendo"-orientation (Fig. 7).
We further identified an immunogenic B-cell epitope at the very C-terminus of M. These results supported the observation that the cytosolic tail and in particular the amino acids between position 195 and 210 of M can serve as an efficient immunogen in antibody production (He et al., 2005; Imgenex 5125A). Obviously, M contains several immunogenic epitopes since sera from SARS patients recognized additional epitopes located in the C- and N-terminal region of M as well [23, 24]. Our attempts to generate antibodies to peptides representing the absolute N-terminus of M, however, were unsuccessful (not shown).
Presented functional analyses showed that the first 134 amino acids of SARS-CoV M comprising the three transmembrane domains are crucial for accumulation of M in the Golgi-complex. Similar findings have been reported for infectious bronchitis virus (IBV), which suggested retention signals in the first transmembrane domain of M . An additional retention signal located in the cytosolic tail is postulated for MHV M . Whereas M of MHV and IBV seem to be retained completely in perinuclear regions , M of TGEV, of the feline coronavirus (FCoV) and of SARS-CoV are also exposed at the plasma membrane [17, 27, 28].
N-glycosylation of SARS-CoV M
Detailed analyses revealed that accumulation of M beyond the budding compartment in the Golgi-complex is a common feature of all investigated coronaviruses [11, 17, 29–31]. In this study we observed that N-glycosylation had no impact on the intracellular distribution of recombinant SARS-CoV M. By utilizing a novel reverse genetic system of SARS-CoV we also did not observe any significant influence of N-glycosylation of M in terms of viability, assembly and budding efficiency and infectivity of recombinant SARS-CoV in cell culture . Supportive for these results are data by de Haan et al., showing that O-glycosylation of murine hepatitis virus (MHV) M is dispensable for assembly and spread of the virus [32, 33]. Most likely the conserved glycosylation of M serves other functions. Previous studies on TGEV revealed an influence of glycosylation of M on interferon induction . In the same direction point experiments in which an artificially introduced N-glycosylation in MHV M caused a strong interferon-α and -β induction while glycosylation-deficient MHV M is a poor interferon-α inducer .
Influence of M on the intracellular distribution of S
Surface spikes composed of the SARS-CoV glycoprotein S confer the name-giving shape of coronaviral particles in electron microscopy analysis . The spikes coat the viral envelope and facilitate interaction of the virus with the cellular receptor and subsequent fusion with the plasma membrane [36–40]. Hence, incorporation of S into the viral envelope is essential for infectivity of released progeny virions [41–43]. A retention signal located at the cytosolic C-terminus of most coronaviral S proteins prevents surface expression of S [44, 45]. However, we and others could observe that upon single expression, S of SARS-CoV is detectable at the plasma membrane [36, 45, 46]. Thus, the question arose whether interaction with other viral proteins supported retention of S. Previous studies on MHV showed an interaction between M and S and emphasized the impact of this interaction on viral morphogenesis [12, 42, 43, 47]. Here, we observed that M suppressed the surface transport of S and may thus functionally support the ER retrieval signal identified by McBride and Machamer . Essential for the retrieval of S was the N-terminus of M.
In summary, by using N-glycosylation mutants and subsequent Endo H digest we could establish a membrane topology model of SARS-CoV M with three transmembrane domains and a Nexo-Cendo-orientation. N-glycosylation of M is neither essential for transport and topology of M nor for its function in replication of recombinant SARS-CoV. Further in vivo studies using the generated N-glycosylation-deficient SARS-CoV are necessary to elucidate the role of conserved N-glycosylation during immune response of the infected host. Finally, our data revealed that the N-terminal part of M induces the retrieval of S to the ERGIC.
Materials and methods
Cells and Viruses
BSR T7/5 cells (BHK-21 cell clone), which constitutively expressed T7 RNA polymerase, were cultured as described by Bucholz et al. . Monolayer cultures of the human hepatoma cell line Huh7 and the African green monkey kidney cell line Vero were grown at 37°C and 5% CO2 in Dulbecco's modified Eagles Medium (DMEM, Gibco) supplemented with 10% fetal calf serum (FCS) 100 U/ml penicillin, and 0.1 mg/ml streptomycin. For the infection of Vero cells SARS-CoV (Frankfurt strain; accession no. AY310120) was used at a multiplicity of infection of approximately 0.1.
Molecular Cloning of M and Glycosylation-Mutants of M
For expression of M and the glycosylation-deficient M N4Q, the respective genes were cloned under the control of the T7 RNA polymerase promoter into the pTM1 plasmid as described previously (Voss et al., 2006). Cloning of the glycosylation mutants MV186N and ML205N was performed using QuickChange site-directed mutagenesis kit (Stratagene) to substitute valine and leucine by asparagine at the indicated amino acid positions. The construction of pTM1-MInsert and pTM1-MN4QInsert was performed by consecutive PCR-steps and subsequent cloning into pTM1 vector. First, fragments encoding amino acids 1–73 and 74–221 of M were amplified using pTM1-M or pTM1-MN4Q as template and the following primers: M1–73 (5'-CGGAATTCATGGCAGACAACGGTACTATTACCG-3' and 5'-acggtagcggttgtatgcgtaattaattctgtagacaacagc-3') and M74–221(5'-gacgatgacaagccaaaagagtgggtgactggcgggattgcg-3' and 5'-CGAGGATCCTTACTGTACTAGCAA AGCAATATTG-3'). In addition, a fragment encoding a stretch of 31 hydrophilic amino acids with a central N-glycosylation site was amplified using the partially complementary oligonucleotides 5'-TACGCATACAACCGCTACCGTATTGGAAACTATAAATTAAATACAACTATTAAGGACCTGGACTACAAGGAC-3' and 5'-CTCTTTTGGCTTGTCATCGTCGTCCTTGTAGTCCAGGTCCTTAATAGTTGTATTTAATTTATAGTTTCC-3'. The three resulting PCR fragments were fused by recombinant PCR leading to MInsert or MN4Q Insert respectively and cloned into the EcoRI and BamHI restriction sites of pTM1 vector. All constructs were verified by restriction analysis and sequencing.
Molecular Cloning of M Truncation Mutants
DNA fragments encoding the C-terminally truncated M mutants were generated by PCR with pTM1-M as template and the following primers: M1–107 (5'- CCGGAATTCATGGACTACAAGGACGACGATGACAAGGCAGACAACGGTACTATTACCGTTG-3' and 5'-CGAGGATCCTTATGAGCGGGTACGAGC-3'); M1–134 (5'-CGGAATTCATGGCAGACAACGGTACTATTACCG-3' and 5'-CGAGGATCCTTACTTGTCATCGTCGTCCTTGTAGTCTTCCATGAGCGGTCTGGTCAC-3'); M1–159 (5'-CGGAATTCATGGCAGACAACGGTACTATTACCG-3' and 5'-CGAGGATCCTTACTTGTCATCGTCGTCCTTGTAGTCGTCACAGCGCCCTAGGGAGTG-3'); M1–189 (5'-CGGAATTCATGGCAGACAACGGTACTATTACCG-3' and 5'-CGAGGATCCTTACTTGTCATCGTCGTCCTTGTAGTCATCAGTGCCTACACGCTGCGAC-3'); MN/C1-189 (5'-CCGGAATTCATGGACTACAAGGACGACGATGACAAGGCAGACAACGGTACTATTACCGTTG-3' and 5'-CGAGGATCCTTACTTGTCATCGTCGTCCTTGTAGTCATCAGTGCCTACACGCTGCGAC-3'); M1–196 (5'-CGGAATTCATGGCAGACAACGGTACTATTACCG-3' and 5'-CGAGGATCCTTACTTGTCATCGTCGTCCTTGTAGTAGTTGTATGCAGCAAAACCTGAATCAGTGCC-3'); M1–201 (5'- CGGAATTCATGGCAGACAACGGTACTATTACCG-3' and 5'-CGAGGATCCTTACTTGTCATCGTCGTCCTTGTAGTCTCCAATACGGTAGCGGTTGTATGCAGC-3'); M1–207 (5'-CGGAATTCATGGCAGACAACGGTACTATTACCG-3' and 5'-CGAGGATCCTTACTTGTCATCGTCGTCCTTGTAGTCTGTATTTAATTTATAGTTTCCAATACGGTAGCGG-3'); M1–212 (5'-CGGAATTCATGGCAGACAACGGTACTATTACCG-3' and 5'-CGAGGATCCTTACTTGTCATCGTCGTCCTTGTAGTCGCTACCGGCGTGGTCTGTATTTAATTTATAG-3'). The fragments were cloned into the EcoRI and BamHI cleavage sites of pTM1. The integrities of all constructs were verified by restriction analysis and sequencing.
Indirect Immunofluorescence Analysis
To perform surface staining of transiently transfected Huh7 cells, cells were grown on glass cover slides and transfected with the plasmids indicated in figure legends as described previously (Voss et al., 2006). At 24 h pt, cells were put on ice and successively incubated with a polyclonal α-FLAG (Sigma) and a secondary rhodamine-coupled α-rabbit antibody (Dianova), respectively. Intracellular staining was achieved by subsequent fixation with 4% PFA/DMEM for 15 min at room temperature, permeabilization with 0.1% Triton X-100 in PBS for 10 min and additional incubation for 1 h with a polyclonal α-FLAG and a FITC-coupled secondary α-rabbit antibody (Dianova) at room temperature. To characterize the influence of M on the intracellular transport of S, subconfluent Huh7 cells were co-transfected with pTM1-MC-FLAG or pTM1-M1–134 and pcDNA.3.1-S (kindly provided by M. Eickmann, Marburg) using FuGENE (Roche) according to the manufactor's protocol. In addition, a plasmid was cotransfected encoding the T7 DNA-dependent RNA-polymerase supporting the expression of the M mutants. At 24 h p.t., the cells were fixed with 4% PFA/DMEM for 15 min and permeabilized for 10 min with 0.1% Triton X-100. Then, unspecific binding sites were blocked using 3% BSA/PBS before the protein of interest was stained using rabbit polyclonal α-FLAG (Sigma) and/or a human monoclonal α-S antibody , respectively. The secondary antibodies used were donkey α-rabbit FITC-coupled and α-human rhodamine-coupled antibody (Dianova). All steps were performed at room temperature. Fluorescence images were taken with fluorescence microscope (Zeiss Axiovert 200M).
Infectious cDNA-clone of SARS-CoV
Engineering of a T7-driven cDNA clone of SARS-CoV has been published previously . Briefly, the viral genome of SARS-CoV (Frankfurt 1 isolate) was subdivided into seven fragments by RT-PCR and subsequently cloned into pCR2.1 (Invitrogen) or pSMART vectors (Lucigen) using appropriate restriction enzymes. In order to achieve a full length SARS-CoV cDNA clone the present constructs were subjected to further restriction/ligation steps into pBelo vectors finally assembling a pBelo-SARS clone under the control of the T7 promoter. The full-length BAC-constructs were linearized, in vitro transcribed and electroporated in BHK-21 cells using GenePulser (Biorad). Harvest and purification of infectious virus was done under BSL4-conditions. According to this approach, we constructed a cDNA-subclone with a substitution in the N-glycosylation site of M by site-directed mutagenesis (Quick change, Stratagene) using wild type pSMART-F as template. Subsequent cloning steps and the rescue of a recombinant SARS-CoV expressing glycosylation-deficient M was performed as described above for wild type recombinant SARS-CoV.
Growth kinetics of recombinant viruses using real time RT-PCR
Vero or FRHK4 cells were infected with plaque-purified wt recSARS-CoV or MN4Q recSARS-CoV with a MOI of 0.05, respectively. At the indicated time points, viral RNA was isolated using QIAamp viral RNA kit according to the manufactor's protocol and subjected to real-time RT-PCR. To monitor viral replication, the viral polymerase gene was amplified using RT PCR . Fluorescence signals were read by LightCycler® (Roche).
Amino acid sequence alignments
In silico protein analysis were performed with the following programs: TMpred, PRODIV, HMMTOP, and tmap.
in vitro translation and subsequent Endo H-digest or immunoprecipitation analyses as well as transient transfection of BHK-T7 cells and Western blot analyses were carried out as described previously .
severe acute respiratory syndrome
transmissible gastroenteritis virus
Enjuanes L, Smerdou C, Castilla J, Anton IM, Torres JM, Sola I, Golvano J, Sanchez JM, Pintado B: Development of protection against coronavirus induced diseases. A review. Adv Exp Med Biol 1995, 380: 197-211.
Tyrrell DA, Cohen S, Schlarb JE: Signs and symptoms in common colds. Epidemiol Infect 1993, 111: 143-156. 10.1017/S0950268800056764
Weiss SR, Navas-Martin S: Coronavirus pathogenesis and the emerging pathogen severe acute respiratory syndrome coronavirus. Microbiol Mol Biol Rev 2005, 69: 635-664. 10.1128/MMBR.69.4.635-664.2005
Watts J: China culls wild animals to prevent new SARS threat. Lancet 2004, 363: 134. 10.1016/S0140-6736(03)15313-5
WHO: Update: Outbreak of severe acute respiratory syndrome – worldwide, 2003. MMWR Morb Mortal Wkly Rep 2003, 52: 241-246.
Drosten C, Gunther S, Preiser W, Werf S, Brodt HR, Becker S, Rabenau H, Panning M, Kolesnikova L, Fouchier RA, et al.: Identification of a novel coronavirus in patients with severe acute respiratory syndrome. N Engl J Med 2003, 348: 1967-1976. 10.1056/NEJMoa030747
Fouchier RA, Kuiken T, Schutten M, van Amerongen G, van Doornum GJ, Hoogen BG, Peiris M, Lim W, Stohr K, Osterhaus AD: Aetiology: Koch's postulates fulfilled for SARS virus. Nature 2003, 423: 240. 10.1038/423240a
Ksiazek TG, Erdman D, Goldsmith CS, Zaki SR, Peret T, Emery S, Tong S, Urbani C, Comer JA, Lim W, et al.: A novel coronavirus associated with severe acute respiratory syndrome. N Engl J Med 2003, 348: 1953-1966. 10.1056/NEJMoa030781
Achonu C, Laporte A, Gardam MA: The financial impact of controlling a respiratory virus outbreak in a teaching hospital: lessons learned from SARS. Can J Public Health 2005, 96: 52-54.
Gupta AG, Moyer CA, Stern DT: The economic impact of quarantine: SARS in Toronto as a case study. J Infect 2005, 50: 386-393. 10.1016/j.jinf.2004.08.006
Klumperman J, Locker JK, Meijer A, Horzinek MC, Geuze HJ, Rottier PJ: Coronavirus M proteins accumulate in the Golgi complex beyond the site of virion budding. J Virol 1994, 68: 6523-6534.
Opstelten DJ, Raamsman MJ, Wolfs K, Horzinek MC, Rottier PJ: Envelope glycoprotein interactions in coronavirus assembly. J Cell Biol 1995, 131: 339-349. 10.1083/jcb.131.2.339
Tooze J, Tooze S, Warren G: Replication of coronavirus MHV-A59 in sac-cells: determination of the first site of budding of progeny virions. Eur J Cell Biol 1984, 33: 281-293.
Armstrong J, Niemann H, Smeekens S, Rottier P, Warren G: Sequence and topology of a model intracellular membrane protein, E1 glycoprotein, from a coronavirus. Nature 1984, 308: 751-752. 10.1038/308751a0
Risco C, Anton IM, Sune C, Pedregosa AM, Martin-Alonso JM, Parra F, Carrascosa JL, Enjuanes L: Membrane protein molecules of transmissible gastroenteritis coronavirus also expose the carboxy-terminal region on the external surface of the virion. J Virol 1995, 69: 5269-5277.
Rottier PJ, Welling GW, Welling-Wester S, Niesters HG, Lenstra JA, Zeijst BA: Predicted membrane topology of the coronavirus protein E1. Biochemistry 1986, 25: 1335-1339. 10.1021/bi00354a022
Voss D, Kern A, Traggiai E, Eickmann M, Stadler K, Lanzavecchia A, Becker S: Characterization of severe acute respiratory syndrome coronavirus membrane protein. FEBS Lett 2006, 580: 968-973. 10.1016/j.febslet.2006.01.026
Nal B, Chan C, Kien F, Siu L, Tse J, Chu K, Kam J, Staropoli I, Crescenzo-Chaigne B, Escriou N, et al.: Differential maturation and subcellular localization of severe acute respiratory syndrome coronavirus surface proteins S, M and E. J Gen Virol 2005, 86: 1423-1434. 10.1099/vir.0.80671-0
Nilsson IM, von Heijne G: Determination of the distance between the oligosaccharyltransferase active site and the endoplasmic reticulum membrane. J Biol Chem 1993, 268: 5798-5801.
Pfefferle S, Grywna K, Drosten C: Characterisation of a patient-derived mutant of SARS-CoV by a novel T7 promoter-driven infectious cDNA clone. Proceedings of the Third European Congress of Virology: 1–5 September 2007; Nuernberg, Germany 2007.
Traggiai E, Becker S, Subbarao K, Kolesnikova L, Uematsu Y, Gismondo MR, Murphy BR, Rappuoli R, Lanzavecchia A: An efficient method to make human monoclonal antibodies from memory B cells: potent neutralization of SARS coronavirus. Nat Med 2004, 10: 871-875. 10.1038/nm1080
Stadler K, Masignani V, Eickmann M, Becker S, Abrignani S, Klenk HD, Rappuoli R: SARS – beginning to understand a new virus. Nat Rev Microbiol 2003, 1: 209-218. 10.1038/nrmicro775
Chow SC, Ho CY, Tam TT, Wu C, Cheung T, Chan PK, Ng MH, Hui PK, Ng HK, Au DM, Lo AW: Specific epitopes of the structural and hypothetical proteins elicit variable humoral responses in SARS patients. J Clin Pathol 2006, 59: 468-476. 10.1136/jcp.2005.029868
He Y, Zhou Y, Siddiqui P, Niu J, Jiang S: Identification of immunodominant epitopes on the membrane protein of the severe acute respiratory syndrome-associated coronavirus. J Clin Microbiol 2005, 43: 3718-3726. 10.1128/JCM.43.8.3718-3726.2005
Swift AM, Machamer CE: A Golgi retention signal in a membrane-spanning domain of coronavirus E1 protein. J Cell Biol 1991, 115: 19-30. 10.1083/jcb.115.1.19
Locker JK, Klumperman J, Oorschot V, Horzinek MC, Geuze HJ, Rottier PJ: The cytoplasmic tail of mouse hepatitis virus M protein is essential but not sufficient for its retention in the Golgi complex. J Biol Chem 1994, 269: 28263-28269.
Jacobse-Geels HE, Horzinek MC: Expression of feline infectious peritonitis coronavirus antigens on the surface of feline macrophage-like cells. J Gen Virol 1983,64(Pt 9):1859-1866. 10.1099/0022-1317-64-9-1859
Laviada MD, Videgain SP, Moreno L, Alonso F, Enjuanes L, Escribano JM: Expression of swine transmissible gastroenteritis virus envelope antigens on the surface of infected cells: epitopes externally exposed. Virus Res 1990, 16: 247-254. 10.1016/0168-1702(90)90051-C
Locker JK, Opstelten DJ, Ericsson M, Horzinek MC, Rottier PJ: Oligomerization of a trans-Golgi/trans-Golgi network retained protein occurs in the Golgi complex and may be part of its retention. J Biol Chem 1995, 270: 8815-8821. 10.1074/jbc.270.15.8815
Machamer CE, Mentone SA, Rose JK, Farquhar MG: The E1 glycoprotein of an avian coronavirus is targeted to the cis Golgi complex. Proc Natl Acad Sci USA 1990, 87: 6944-6948. 10.1073/pnas.87.18.6944
Rottier PJ, Rose JK: Coronavirus E1 glycoprotein expressed from cloned cDNA localizes in the Golgi region. J Virol 1987, 61: 2042-2045.
de Haan CA, de Wit M, Kuo L, Montalto-Morrison C, Haagmans BL, Weiss SR, Masters PS, Rottier PJ: The glycosylation status of the murine hepatitis coronavirus M protein affects the interferogenic capacity of the virus in vitro and its ability to replicate in the liver but not the brain. Virology 2003, 312: 395-406. 10.1016/S0042-6822(03)00235-6
de Haan CA, Kuo L, Masters PS, Vennema H, Rottier PJ: Coronavirus particle assembly: primary structure requirements of the membrane protein. J Virol 1998, 72: 6838-6850.
Laude H, Gelfi J, Lavenant L, Charley B: Single amino acid changes in the viral glycoprotein M affect induction of alpha interferon by the coronavirus transmissible gastroenteritis virus. J Virol 1992, 66: 743-749.
Almeida JD, Tyrrell DA: The morphology of three previously uncharacterized human respiratory viruses that grow in organ culture. J Gen Virol 1967, 1: 175-178. 10.1099/0022-1317-1-2-175
Simmons G, Reeves JD, Rennekamp AJ, Amberg SM, Piefer AJ, Bates P: Characterization of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) spike glycoprotein-mediated viral entry. Proc Natl Acad Sci USA 2004, 101: 4240-4245. 10.1073/pnas.0306446101
Hofmann H, Hattermann K, Marzi A, Gramberg T, Geier M, Krumbiegel M, Kuate S, Uberla K, Niedrig M, Pohlmann S: S protein of severe acute respiratory syndrome-associated coronavirus mediates entry into hepatoma cell lines and is targeted by neutralizing antibodies in infected patients. J Virol 2004, 78: 6134-6142. 10.1128/JVI.78.12.6134-6142.2004
Li W, Moore MJ, Vasilieva N, Sui J, Wong SK, Berne MA, Somasundaran M, Sullivan JL, Luzuriaga K, Greenough TC, et al.: Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus. Nature 2003, 426: 450-454. 10.1038/nature02145
Frana MF, Behnke JN, Sturman LS, Holmes KV: Proteolytic cleavage of the E2 glycoprotein of murine coronavirus: host-dependent differences in proteolytic cleavage and cell fusion. J Virol 1985, 56: 912-920.
Follis KE, York J, Nunberg JH: Furin cleavage of the SARS coronavirus spike glycoprotein enhances cell-cell fusion but does not affect virion entry. Virology 2006, 350: 358-369. 10.1016/j.virol.2006.02.003
Huang Y, Yang ZY, Kong WP, Nabel GJ: Generation of synthetic severe acute respiratory syndrome coronavirus pseudoparticles: implications for assembly and vaccine production. J Virol 2004, 78: 12557-12565. 10.1128/JVI.78.22.12557-12565.2004
Nguyen V-P, Hogue BG: Protein interactions during coronavirus assembly. J Virol 1997, 71: 9278-9284.
Opstelten DJ, Horzinek MC, Rottier PJ: Complex formation between the spike protein and the membrane protein during mouse hepatitis virus assembly. Adv Exp Med Biol 1993, 342: 189-195.
Lontok E, Corse E, Machamer CE: Intracellular targeting signals contribute to localization of coronavirus spike proteins near the virus assembly site. J Virol 2004, 78: 5913-5922. 10.1128/JVI.78.11.5913-5922.2004
Schwegmann-Wessels C, Al-Falah M, Escors D, Wang Z, Zimmer G, Deng H, Enjuanes L, Naim HY, Herrler G: A novel sorting signal for intracellular localization is present in the S protein of a porcine coronavirus but absent from severe acute respiratory syndrome-associated coronavirus. J Biol Chem 2004, 279: 43661-43666. 10.1074/jbc.M407233200
McBride CE, Li J, Machamer CE: The cytoplasmic tail of the severe acute respiratory syndrome coronavirus spike protein contains a novel endoplasmic reticulum retrieval signal that binds COPI and promotes interaction with membrane protein. J Virol 2007, 81: 2418-2428. 10.1128/JVI.02146-06
de Haan C, Smeets M, Vernooij F, Vennema H, Rottier P: Mapping of the coronavirus membrane protein domains involved in interaction with the spike protein. J Virol 1999, 73: 7441-7452.
Buchholz UJ, Finke S, Conzelmann KK: Generation of bovine respiratory syncytial virus (BRSV) from cDNA: BRSV NS2 is not essential for virus replication in tissue culture, and the human RSV leader region acts as a functional BRSV genome promoter. J Virol 1999, 73: 251-259.
Kolesnikova L, Berghofer B, Bamberg S, Becker S: Multivesicular bodies as a platform for formation of the Marburg virus envelope. J Virol 2004, 78: 12277-12287. 10.1128/JVI.78.22.12277-12287.2004
The authors wish to thank Angelika Lander and Ulla Thiesen for their expert technical assistance and Larissa Kolesnikova for EM studies. This work was supported by the European Union FP6 SARSVAC.
The authors declare that they have no competing interests.
DV designed the study, conducted the experiments and wrote the manuscript. DV and SP carried out the experiments in BSL-4 facility. LS constructed mutants and helped to carry out the biochemical studies. CD, ET and AL contributed materials and/or critically revised the manuscript. SB, designed and coordinated the study, participated in the analysis of the results and drafted the manuscript. All authors read and approved the final manuscript.
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About this article
- Severe Acute Respiratory Syndrome
- Infectious Bronchitis Virus
- Perinuclear Region
- Severe Acute Respiratory Syndrome
- Membrane Topology