Evidence for a novel gene associated with human influenza A viruses

Table 2 Nucleotide positions (aligned) of fixed mutations in human influenza A virus genomes

Position	NS1 protein	NEG8 reading frame	NS2 protein
183	N>D	F>S
189	E>K	S>L
202	R>H	R>W
215	no change	no change
218	no change	no change
221	no change	P > L
226	R>K	L>F
245	no change	no change
271	A>V	A>T
326	no change	no change
327	N>D	S>F
341	no change	M>I
361	A>E	A>S
365	no change	no change
374	no change	I>M
383	I>M	no change
392	no change	no change
401	D>E	L>F
404	no change	no change
413	I>M	no change
414	no change	S>N
437	no change	no change
449	no change	no change
455	no change	no change
456	L>I	R>M
459	I>V	I>T
506	no change	no change
522	L>F	R>K
538	N>I	L>I
616	L>I	V>F
636	no change	P > L
657	no change	L>P	no change
668	S>L	no change	S>L
702	V>I	I>T	no change
703	I>A	T>A	no change
723	N>D	F>S	no change

Of 36 fixed single-nucleotide mutations in the avian influenza A viruses in the region of genome segment 8 spanning the region equivalent to the NEG8 ORF, 11 had no effect on NS1, NS2 or NEG8 reading frames, 17/36 had no effect on NS1, 4/5 had no effect on NS2. 22/36 mutations resulted in a change in the predicted amino acid sequence of the NEG8 protein

ISSN: 1743-422X