Table 2 The oligonucleotide primers, amplicon length and amplification efficiency of the real time RT-PCR primers amplifying IFN, Mx, 18S, ISG15 and STAT1 genes
Primer name | Primer sequence | Amplicon length (bp) | Amplification efficiency |
|---|---|---|---|
As IFN Fwd1 | TGCAGTATGCAGAGCGTGTG | 100 | 1.83 |
As IFN Rev1 | TCTCCTCCCATCTGGTCCAG | Â | Â |
As Mx Fwd | TGCAACCACAGAGGCTTTGAA | 77 | 1.88 |
As Mx Rev | GGCTTGGTCAGGATGCCTAAT | Â | Â |
As 18S Fwd | TGTGCCGCTAGAGGTGAAATT | 60 | 1.86 |
As 18S Rev | GCAAATGCTTTCGCTTTCG | Â | Â |
As ISG15 Fwd | CTGAAAAACGAAAAGGGCCA | 100 | 1.83 |
As ISG15 Rev | GCAGGGACTCCCTCCTTGTT | Â | Â |
As STAT1 Fwd | TGTCTGTTGGCTCAGTTGCG | 100 | 1.82 |
As STAT1 Rev | GAAATTGATGCTGTGGCGTCT | Â | Â |
As PKZ Fwd | AGATAGCGAAGGCTGTTGGA | 101 | 1.913 |
As PKZ Rev | TGGTTTGTCTGGTGTTGCAT | Â | Â |