- Open Access
Avian leukosis virus subgroup J associated with the outbreak of erythroblastosis in chickens in China
- Guihua Wang†1, 2,
- Yanping Jiang†1,
- Linin Yu1,
- Yue Wang1,
- Xiaomin Zhao1, 2 and
- Ziqiang Cheng1, 2Email author
© Wang et al.; licensee BioMed Central Ltd. 2013
- Received: 2 November 2012
- Accepted: 11 March 2013
- Published: 22 March 2013
Emaciation, depression and lethargy were observed in two flocks of Chinese local breed and one flock of commercial layer chicken infected naturally from 2010 to 2011. The aims of this study were to diagnose.
Methods and results
Gross observation showed that severe enlargement of liver, spleen and kidney, and hemorrhage of thymus, muscle and glandular stomach in all submitted birds. The liver and lung of one flock had diffuse, multifocal white raised foci on the surface as well as on the cut-surface. Numerous erythrocytoblasts with bigger volume, basophilic cytoplasm and round nucleus were observed in blood and bone marrow smears. The same erythrocytoblasts were also found crowded in blood vessels and mesenchym of tissues by histological examination, and some had mitotic figures. PCR results showed that three flocks were positive for ALV-J with specific fragment of 924 bp, negative for AEV, ALV-A, ALV-B, Marek’s disease virus (MDV) and Reticuloendotheliosis virus (REV). The results of immunohistochemistry showed that cytoplasm of histiocytes and erythrocytoblasts in lung and spleen sections was positive for ALV-J antigen.
These data demonstrated that erythroblastosis was all induced by ALV-J in the three different flocks. This is the first document report of erythroblastosis induced by ALV-J in China flocks.
- Avian leukosis virus subgroup J
Avian leukosis virus subgroup J (ALV-J) is an oncogenic exogenous retrovirus first isolated in the late 1980s and reported in 1991 . The hosts with clinical infection of ALV-J are characterized as immune tolerance, high mortality, delayed growth, and development of a variety of tumors including myelocytomas, sarcomas, hemangiomas, nephromas and erythroblastosis [2–5]. The erythroblastosis is a neoplastic disease induced by viral disoperation for erythroblast in bone marrow. In 1988, Houghton et al. found that the chicken erythroblastosis was associated with ALV-J during their investigation of the neoplastic disease of broilers with experimental infections of ALV-J . Venugopal et al. (2000) observed the indicative lesions of erythroblastosis in tissues from flocks with suspicion of ALV-J infection. However, the chicken erythroblastosis induced by ALV-J has never been identified in China.
In the present study, we identified the chicken erythroblastosis that was associated with natural infections of ALV-J in two flocks of Chinese local breed and one flock of commercial layer chicken from 2010 to 2011. This is the first report of the chicken erythroblastosis induced by ALV-J in China.
This study was carried out in strict adherence to the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Healthy. The protocol was approved by the Committee on the Ethics of Animal of Shandong (Permit Number: 20100326).
From the year 2010 to 2011, our laboratory (The Molecular Pathology Laboratory, College of Veterinary Medicine, Shandong Agricultural University) received six sick representatives of the 20-day-old commercial layer chickens (flock 1), three 90-day-old (flock 2) and three 110-day-old (flock 3) Chinese local breed chickens for the diagnostic purpose. The birds of flock 1 presented depression, recumbency and pale cockscomb started from 15 days of age, and the mortality in the population was 18%. The birds of flock 2 showed symptoms of nerve system disorders such as depression and ataxia started from 85-day-old, and the mortality was 12%. The birds of flock 3 anorexia, lethargy and emaciation started from 90-day-old, and the mortality reached 20% at 100-day-old.
The samples of the liver, spleen, kidney, heart, lung, proventriculus, sciatic nerve, brain, and bone marrow were collected and fixed in 10% buffered neutral formalin. The fixed tissues were embedded in paraffin, sectioned at 4 μm thick, and stained with haematoxylin and eosin. The sample slides were observed under light microscopy.
Polymerase chain reaction (PCR)
Primers for differential diagnosis
R :5′- AAAGACCGATGCCTAGACCAACC-3′
F :5′ –CGAGAGTGGCTCGCGAGATGG-3′
To detect the presence of ALV-J and AEV antigen, tissues were fixed with 10% buffered neutral formalin, paraffin-embedded, sectioned with the thickness of 4 μm, and mounted on poly-l-lysine-coated slides. The tissue sections were stained with a routine streptavidin biotin/horseradish peroxidase (HRP)-conjugated immunohistochemical technique as described by . Briefly, the sections were pre-treated with 3% hydrogen peroxide in methanol, and blocked with 5% bovine serum albumin in PBS for 10 min. Then the slides were incubated with primary antibody (a rabbit anti-ALV-J and anti-AEV surface protein prepared by our lab) at a dilution of 1: 400 for 1 h, washed three times with PBS, and incubated with the secondary antibody (biotinylated goat anti-rabbit IgG, Santa Cruz, CA, USA) at a dilution of 1:5000 for 30 min. After three washes, the tertiary conjugate streptavidin/HRP was applied for 30 min. Chromogen (AEC) was applied and developed microscopically for positive straining. The reaction was stopped by water and the slides were then counterstained with hematoxylin. Finally, the slides examined microscopically with light microscopy. In negative immunostaining controls, the primary antibody was replaced with non-immune rabbit IgG.
Antigen distribution and tropism
The findings in the present paper documented the occurrence of ALV-J-induced erythroblastosis in commercial layer chickens and Chinese local breed chickens. Numerous erythroblasts at different growth stages in the blood, spleen, lung, bone marrow and other organs of the infected birds were observed consistently. Neoplastic lymphocytes were not observed. Thus, Marek’s disease and reticuloendotheliosis were all eliminated through examination of hematology and histology. The PCR results further supported this conclusion.
Several ALV strains have been reported to induce erythroblastosis. These included chronic ALV strains such as RPL12  and RAV-1  that induce erythroblastosis by the activation of cellular oncogene c-erbB by LTR insertion  and acutely transforming viruses such as AEV-H and AEV-ES4 strains containing erb-A and/or erb-B oncogenic sequences . To identify the possible viral pathogens of the sick chickens, in the present study we designed primers specific to AEV genes encoding polyproteins gag-p75-erbA and erbB based on the published sequence (Genbank access number: K02006.1) for PCR assays. The negative PCR and immunohistochemistry results of all the tested samples using the AEV specific primers eliminate the infection of AEV in the examined chickens. With the same philosophy and method, we also eliminated the ALV-A and ALV-B infections in the examined chickens. The most PCR reactions of the tested samples (10/12) are positive when using the ALV-J specific primers. Token together, the results of the viral specific PCR assays suggest that the examined chickens were infected with ALV-J.
Disease associated with ALV-J has, since its reported in the last century 90’s , become a major problem in chickens worldwide associated with the high oncogenicity and broad carcinoma spectrum. ALV-J predominantly induces a late-onset myelocytomatosis  because of their tropism to the cells of the myeloid rather than the lymphoid lineage . During the last 2 years, we have observed, in addition to myelocytomatosis, the occurrence of neoplastic lesions which are the indicative of erythroblastosis in various tissues of chickens from three flocks naturally infected with ALV-J. The results of immunohistochemistry demonstrated that the extension of this tropism of ALV-J strains infected submitted birds for cells of erythroid lineage in vivo. This is the first time that erythroblastosis have been identified as the primary neoplastic lesion induced by ALV-J in China.
Clinically, the erythroblast leukemia is divided into two types: anemia and hyperplasia. The hyperplasia type characterized with the presence of massive erythroblasts in blood was more common than anemia type characterized with rare immature erythrocytes. In this case, significantly increased erythroblasts were observed in histopathological sections of all submitted birds, by which hyperplasia type of erythroblast leukemia was diagnosed.
Venugopal et al. have reported that the incidence of erythroblastosis was higher in birds inoculated with the virus after hatching, and it is possible that the transformation of erythroblasts could be dependent on the developmental stage and numbers of the target cells at the time of infection . In this study, the incubation periods of two flocks of Chinese local breed were similar and longer than that of commercial layer chickens. Unfortunately, the time of infection was not clear. The susceptivity of host was maybe an important factor.
This research was supported by the National Natural Science Foundation of China (31072096) and the Ministry Education New Teacher Foundation of China (20113702120005).
- Payne L, Brown S, Bumstead N, Howes K, Frazier JA, Thouless ME: A novel subgroup of exogenous avian leukosis virus in chickens. J Gen Virol 1991, 72: 801-807. 10.1099/0022-1317-72-4-801PubMedView ArticleGoogle Scholar
- Arshad S, Howes K, Barron G, Smith L, Russell P, Payne L: Tissue tropism of the HPRS-103 strain of J subgroup avian leukosis virus and of a derivative acutely transforming virus. Vet Pathol Online 1997, 34: 127-137. 10.1177/030098589703400205View ArticleGoogle Scholar
- Fadly A, Payne L: Leukosis/sarcoma group. Diseases of poultry. 11th edition. Iowa: Iowa State Press; 2003:465-516.Google Scholar
- Sironi G, Manarolla G, Pisoni G, Recordati C, Rampin T: Myotropic avian leukosis virus subgroup J infection in a chicken. J Vet Med B 2006, 53: 347-349. 10.1111/j.1439-0450.2006.00961.xView ArticleGoogle Scholar
- Stedman N, Brown T: Body weight suppression in broilers naturally infected with avian leukosis virus subgroup J. Avian Dis 1999, 43: 604-610. 10.2307/1592664PubMedView ArticleGoogle Scholar
- Payne L: Retrovirus-induced disease in poultry. Poult Sci 1998, 77: 1204-1212.PubMedView ArticleGoogle Scholar
- Silva RF, Fadly AM, Taylor SP: Development of a polymerase chain reaction to differentiate avian leukosis virus (ALV) subgroups: detection of an ALV contaminant in commercial Marek’s disease vaccines. Avian Dis 2007, 51: 663-667. 10.1637/0005-2086(2007)51[663:DOAPCR]2.0.CO;2PubMedView ArticleGoogle Scholar
- Smith EJ, Williams SM, Fadly AM: Detection of avian leukosis virus subgroup J using the polymerase chain reaction. Avian Dis 1998, 42: 375-380. 10.2307/1592488PubMedView ArticleGoogle Scholar
- Aly MM, Smith EJ, Fadly AM: Detection of reticuloendotheliosis virus infection using the polymerase chain reaction. Avian Pathol 1993, 22: 543-554. 10.1080/03079459308418942PubMedView ArticleGoogle Scholar
- Silva R, Smith E: PCR as a tool for the diagnosis of avian tumor viruses and tumors. In Proc. Avian Tumor Virus Symposium. Reno, NV: American Association of Avian Pathologists; 1997:19-22.Google Scholar
- Crespo R, Woolcock PR, Fadly AM, Hall C, Shivaprasad H: Characterization of T-cell lymphomas associated with an outbreak of reticuloendotheliosis in turkeys. Avian Pathol 2002, 31: 355-361. 10.1080/03079450220141624PubMedView ArticleGoogle Scholar
- Fredrickson T, Piraino F, Okazaki W, Burmester B: Responses of different stocks of chickens to inoculation as embryos and as chicks with strain RPL 12 and field isolates of leukosis virus. Avian Dis 1964, 8: 123-134. 10.2307/1587828View ArticleGoogle Scholar
- Nilsen TW, Maroney PA, Goodwin RG, Rottman FM, Crittenden LB, Raines MA, Kung HJ: c-erbB activation in ALV-induced erythroblastosis: novel RNA processing and promoter insertion result in expression of an amino-truncated EGF receptor. Cell 1985, 41: 719-726. 10.1016/S0092-8674(85)80052-0PubMedView ArticleGoogle Scholar
- Fung Y, Lewis WG, Crittenden LB, Kung HJ: Activation of the cellular oncogene c-erbB by LTR insertion: molecular basis for induction of erythroblastosis by avian leukosis virus. Cell 1983, 33: 357-368. 10.1016/0092-8674(83)90417-8PubMedView ArticleGoogle Scholar
- Hayman M, Beug H: Avian erythroblastosis: a model system to study oncogene co-operation in leukemia. Cancer Surv 1992, 15: 53-68.PubMedGoogle Scholar
- Payne NL, K H, Gillespie MA, Smith ML: Host range of Rous sarcoma virus pseudotype RSV (HPRS-103) in 12 avian species: support for a new avian retrovirus envelope subgroup, designated J. Soc Gen Microbi 1992, 73: 2995-2997.Google Scholar
- Payne L, Gillespie A, Howes K: Myeloid leukaemogenicity and transmission of the HPRS-103 strain of avian leukosis virus. Leukemia 1992, 6: 1167-1176.PubMedGoogle Scholar
- Venugopal K, Howes K, Flannery D, Payne L: Isolation of acutely transforming subgroup J avian leukosis viruses that induce erythroblastosis and myelocytomatosis. Avian Pathol 2000, 29: 497-503. 10.1080/030794500750047252PubMedView ArticleGoogle Scholar
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