Comparative analysis of the genome sequences and replication profiles of chikungunya virus isolates within the East, Central and South African (ECSA) lineage
© Chen et al.; licensee BioMed Central Ltd. 2013
Received: 4 December 2012
Accepted: 28 May 2013
Published: 30 May 2013
A comparative analysis of the genomic and replication profiles of different geographical chikungunya virus (CHIKV) isolates of the East, Central and South African (ECSA) lineage was performed.
Analysis of the data revealed the different growth kinetics for the different isolates. Deep genome sequencing analysis further revealed specific amino acid mutations in the viral nsP1, nsP3, nsP4, E1 and E2 proteins in the different isolates. Despite the difference in viral genomic profiles, the virus-induced ultrastructural changes within infected cells remained highly conserved among the different chikungunya virus isolates.
These findings provide insights into the genomic and replication profiles of the re-emerging chikungunya virus isolates of the ECSA lineage.
KeywordsChikungunya virus Genome sequencing Virus morphogenesis Virus replication kinetics
Chikungunya virus (CHIKV) was first isolated following an outbreak in Tanzania in the 1950s . CHIKV infection typically induced an acute onset of painful syndrome as characterised by polyarthralgia, fever, asthenia, headache, myalgia and skin rashes [2, 3].
CHIKV is an enveloped virus that possesses a single-stranded, positive-sense RNA genome. The 12Kb viral genome begins with an UTR at the 5’-terminal and followed by two-third of the coding regions for the non-structural proteins (nsP1-4), and the remaining one-third of the coding regions encodes for the structural proteins (Capsid-E3-E2-6K-E1), and a 3’-terminal poly-A-tail . The non-structural proteins are processed into four nsPs and responsible for different roles in virus replication. nsP1 is important in viral RNA synthesis initiation and viral RNA capping . nsP2 possesses protease and RNA helicase activities [6, 7]. nsP3 is composed of three domains and this viral protein is required for the formation and localization of replication complexes [7, 8]. nsP4 possesses RNA-dependent RNA polymerase (RdRp) activity that is important for replication and synthesis of the viral genome [8, 9].
The urban transmission of the CHIKV involves the naive human host and the Aedes mosquito, with Aedes aegypti and Aedes albopictus as the main vectors . Although the mortality rate is rather low, the epidemic potential of the virus is rather fearful due to the prolonged morbidity and the rapid spread of the virus. In 2004, the virus managed to catch fresh attention when it re-emerged and set its foot around the world. The first wave started in Kenya, the re-emergence of the virus was then reported in countries around the Indian Ocean [11, 12]. From 2006 onwards, more countries including India and Sri Lanka have also reported severe outbreaks of the CHIKV infections . In addition, outbreaks of CHIKV were also documented in South East Asia, with countries such as Malaysia, Thailand and Singapore being affected .
Phylogenetic analysis of CHIKV genome revealed three lineages namely: Asian, West African (WA) and East, Central and South African (ECSA) . Viral genome sequence analysis revealed that the CHIKV isolates from the recent outbreaks in the India Ocean and South East Asia were predominantly attributed by the ECSA lineage [12, 15, 16]. In addition, detailed phylogenetic analysis further identified two distinct sub-lineages in the ECSA clades between the outbreaks in Indian Ocean island and India . The CHIKV outbreak in Indian Ocean, in particularly island of La Reunion, carries a distinct substitution of alanine for valine at the 226 position of the CHIKV E1 protein. This E1:A226V substitution has been suggested to allows a better adaptation of the virus in the Aedes albopictus as compared to classical vector, Aedes aegypti, which leads to a rapid dissemination of the virus in La Reunion . In addition, the re-emergence CHIKV outbreak occurring in the South East Asia was determined to be more related to the Indian sub-lineage of ECSA as compared to the Indian Ocean .
Singapore had it first outbreak with CHIKV in the year of 2008 [18, 19]. In 2008 alone, several outbreaks have occurred in Singapore with the first outbreak occurring in January 2008 and genome sequencing of the CHIKV isolate revealed that the virus does not harbour the distinct E1:A226V mutation. The subsequent episodes of outbreaks in Singapore were caused by the CHIKV with the distinct E1:A226V mutation . Here, in this study, we are interested in the comparative analysis of the genomic sequences and replication profiles of the different Singapore CHIKV isolates and the La Reunion (IMT) CHIKV isolate that are belonging to ECSA lineage. The first CHIKV isolate, SGP007 is a wild type E1:226A CHIKV isolated from patient in Singapore in 2008. The second CHIKV isolate SGP011, contains the E1:A226V mutation and was also isolated from patient during the Singapore outbreak in 2008 . The third isolate, La Reunion (IMT) was isolated from the outbreak in La Reunion that contains the E1:A226V mutation . All three isolates used were of low passage numbers upon isolation from patients.
We then proceeded to detect the presence of CHIKV antigens using flow cytometry in an effort to further define the virus replication kinetics. CHIKV-infected cells were fixed and permeabilised (BD Bioscience) before being stained with an anti-alphavirus Ab (10 μg/ml, Santa Cruz) for 30 min, followed by APC-conjugated goat anti-mouse IgG F(ab)2 Ab (10 μg/ml, Invitrogen) for 30 min. Data was acquired in BD FACS Canto II (BD Biosciences) using BD FACSDiva software. In both C6/36 and HeLa cell lines, IMT and SGP011 isolates demonstrated similar infectivity kinetics and both isolates showed a significantly higher viral antigen staining compared to SGP007 (Figure 1e and f). This suggested that both IMT and SGP011 exhibited higher virus infectivity and viral antigen production in the infected cells when compared to SGP007 isolate.
To better understand if the differences in replication kinetics of the different CHIKV isolates were associated with specific mutation(s) in the viral genomes, viral RNA was isolated from each CHIKV isolate and reverse-transcribed into cDNA and sent for sequencing. The three cDNA libraries were sequenced as a multiplex in a single lane using the Illumina HiSeq2000 (Next Generation Sequencing Core facility, Genomic Institute of Singapore) at a coverage of 67436×, representing about 20 million reads per sample. De novo assembly and mapping of each sequence was performed using the velvet assembler and bowtie enabled by Pipeline Pilot, with hash size = 31 and insert size = 150. All three sequences seemed to be missing 20–50 bp from the 3' and 5' ends when compared to the prototype isolate (S27 African prototype). In order to perform end-repair, 100 000 reads were mapped with bowtie to the prototype isolate, and some of the end sequences were recovered. The libraries were compared with the ERCC spike-in RNA controls (Invitrogen), and all three libraries had a > 0.99 correlation between normalised read counts and the control concentrations in a log scale.
When compared to the CHIKV S27 African prototype isolate , we identified seven nucleotide (nt) substitutions within the genome of the SGP007 CHIKV isolate that resulted in amino acid (aa) changes (Additional file 1: Table S1). S27 African prototype and SGP007 CHIKV isolates had identical amino acid residues at various positions (nsP3 aa524, nsP4 aa75 and E1 aa226). The E1 A226V mutation suggested a closer identity between these two isolates. However, SGP007 CHIKV obtained an extra unique mutation at various positions (nsP1 aa60, nsP3 aa137, nsP4 aa563 and E2 aa178). It remains unknown whether these mutations will support the unique feature of SGP007 CHIKV in terms of virus replication. We did not identify any unique mutations within the nsP2, Capsid, E3 or 6K protein sequences.
Till now, there is limited information available regarding the relationship between the unique amino acid residues and the functions of alphavirus nsPs. Previous studies have suggested the role of E1 A226V mutation in the fitness of CHIKV in Aedes aegypti and Aedes albopictus mosquitoes . Further studies have also suggested the role of E2 L210Q mutation in the sequential adaptive mutations in CHIKV and enhancement of efficient vector switching in nature . More amino acid positions within the CHIKV structural proteins have been demonstrated to show positive selection effect in virus circulation and persistence in endemic areas . Here, we would like to postulate the role and effect of amino acid mutations in the nsPs on virus replication efficiency.
We first identified one unique mutation in SGP007 (nsP3 A137V) located within the macro domain region (Additional file 1: Table S1). The first 160 amino acids of the N-terminal region of nsP3 are known as the macro domain, which possesses ADP-ribose 1”-phosphate phosphatase activity. In addition, a study using Sindbis virus suggested an important role of the nsP3 macro domain in virus replication . However, structural mapping suggested that this amino acid is not positioned within the enzymatic groove of the protein (data not shown). Therefore, it is unlikely that mutations at this position would alter the phosphatase activity and further studies would be needed to investigate the importance of this mutation (nsP3 A137V) in CHIKV virus replication.
We further suggested a possible role for the opal stop codon that located at the C-terminal of the nsP3 proteins in enhancing CHIKV virus replication in vitro. Several alphaviruses express two different isoforms of nsP3, depending on the presence of an opal stop codon or an open reading frame upstream of nsP4 (7 amino acids before the C-terminal of the nsP3) [27, 28]. Expression of CHIKV nsP3 in different isoforms with different properties or functions remains to be further characterized. CHIKV S27 African prototype and SGP007 have an identical amino acid residue at nsP3 position 524 (arginine), but in IMT and SGP011, this position encodes a stop codon instead. In vitro infection results showed that SGP007 resulted in lower virus replication efficiency when compared to IMT and SGP011 (Figure 1). It was shown previously that the Opal stop codon at this particular position improved O’Nyong-Nyong virus infectivity, relative to viruses encoding an arginine at the corresponding position . In addition, a study using Semliki Forest virus also showed improved nsP3 protein stability after removing the nsP3 C-terminal region . Therefore, our results are consistent with previous findings from other alphaviruses that CHIKV isolates possessing an opal stop codon upstream of nsP4 could improve nsP3 protein stability and virus infectivity. This in turn could enhance virus replication. In addition, the T75A amino acid residue at the nsP4 N-terminal region was found to correlate with the nsP3 C-terminal region in regulating CHIKV viral replication.
One last unique mutation identified from CHIKV SGP007 was the I563T amino acid residue at nsP4 located within the RdRp region. Within the structural region, one unique mutation identified from CHIKV SGP007 was the R178H amino acid residue at E2 glycoprotein and is located within the E2 domain B region. However, the precise role of the differences in these amino acids in the various CHIKV isolates in virus replication remains undefined.
To further address the issues if the specific amino acids differences between the different CHIKV isolates will affect the formation and morphogenesis of virus-induced structures during CHIKV replication within cells, we utilised transmission electron microscope (TEM) as a tool to decipher these replication processes within the infected cells. The replication process of CHIKV has not been well-defined and many studies are still based on the assumption on other established alphaviruses. In this part of the study, the C6/36 cells were infected with CHIKV at the MOI of 10 and morphological changes were observed and documented under TEM at different time points post-infection. Similarly, the HeLa cells were infected with CHIKV at the same MOI and processed for the ultrastructural analysis at different time points post-infection with the different isolates of CHIKV.
Generally, upon infection with the different CHIKV isolates, the virus induced structures and morphogenesis observed were highly conserved. The first distinct viral-induced structures observed, were the presence of the cytopathic vacuoles type I (CPV I) complexes. This occurred as early as 6 to 8 hours post-infection. These distinct vacuoles measured to be approximately 800 nm to 2000 nm in diameter and are characterized by the presence of membranous spheres lining on the vacuoles (Figure 2b). These membranous spheres (Figure 2b, arrows) measured to be 60 nm to 80 nm in sizes and were seen to be neatly arranged on the vacuoles. These vacuoles were also documented in cells infected with other alphaviruses and these vacuoles were proven to be the replication complexes where viral RNA synthesis occur and were seen in the early infection . Other studies also showed the presence of the viral replicases in CPV I complexes of other alphaviruses. The staining of the cellular markers for the cytoplasmic endosome and lysosome suggested the origin of these CPV I complexes [31, 32]. As the infection progressed to 10 hours post-infection, the degenerated forms of CPV I can be observed. The membranous spheres within the CPV I complexes are no longer visible and are replaced by vacuoles containing bags of membranous whorls (data not shown).
The appearance of the CPV I complex at 8 hours post-infection was accompanied by the emergence of the mature virus particles budding at the plasma membrane (Figure 2c). The viral nucleocapsids of the CHIKV measured approximately 30 nm to 40 nm in size can be seen to be lining in cytoplasm, underneath the plasma membrane (Figure 2ci). The viral nucleocapsids were seen to be expelled out of the cytoplasm through blebbing and pinching off the plasma membrane (Figure 2d), acquiring a portion of the membrane as its envelope (Figure 2di). The full mature virus was measured to be approximately 60 nm to 70 nm in size. The progression of the infection then saw an array of neatly arranged mature virus lining in rows on the peripheral of cells and in between the junction of two adjacent cells. This mode of virus release from the infected cells via budding through the plasma membrane occurred at early stages of infection and marked the first mode of viral release.
As the infection progressed, the dominant viral-induced structure was replaced by the cytopathic vacuoles type II (CPV II) complexes. This phenomenon occurred as early as 24 hours post-infection. Unlike the CPV I, these CPV II complexes were characterized by smaller vacuoles, measuring 300 nm to 400 nm in size (Figure 2e). These complexes were often observed in large number and cluster near the plasma membrane (Figure 2f). The cytoplasmic side of the CPV II vacuoles was surrounded by neatly arranged electron-dense viral nucleocapsids measuring 30 nm to 40 nm in size. These nucleocapsids can be observed to be budding into the CPV II complexes, and were thought to mature in these CPV II vacuoles. Subsequently, these CPV II vacuoles can be seen to contain full matured enveloped CHIKV viruses (Figure 2f, arrow), and the fusion of these vacuoles with the plasma membrane to release the matured virus out of the cell (Figure 2g). As such, it can be postulated that the CPV II may function as exocytosis vesicles or vacuoles for the release of the CHIKV particles at late infection. In addition, crystalline array of virus particles accumulating on the surface of the infected cells can be seen (Figure 2h). This mode of virus release through exocytosis of the CPV II complex with the fusion with the plasma membrane marked the second mode of viral release during the late stages of infection and may provide a means of rapid release of the mature viruses to the exterior of cell before cell death sets in.
The re-emergence of CHIKV since 2004 has brought in much attention to this once neglected virus. This study has documented the differences in the replication kinetics of the different CHIKV isolates within the ECSA lineage. Despite the differences in the replication kinetics, the virus induced ultrastructural changes and virus morphogenesis remains highly conserved between the different CHIKV isolates of the ECSA lineage.
We thank Diane Simarmata (SIgN) for technical assistance in the virus infections. We also, thank Josephine Lum and Francesca Zolezzi (SIgN) for their technical assistance in the deep sequence analysis, and Micheal Poidinger (SIgN) for bioinformatics data analysis.
This work is supported by grant from Biomedical Research Council, A*STAR, Grant No: 09/1/12/19/596 (R182-000-188-305) and National University of Singapore, Start-up grant (R182-000-165-133, R182-000-165-733).
- Ross RW: The Newala epidemic. III. The virus: isolation, pathogenic properties and relationship to the epidemic. J Hyg (Lond) 1956,54(2):177-191. 10.1017/S0022172400044442View ArticleGoogle Scholar
- Gérardin P, et al.: Estimating chikungunya prevalence in La réunion island outbreak by serosurveys: two methods for two critical times of the epidemic. BMC Infect Dis 2008,28(8):p99.View ArticleGoogle Scholar
- Sissoko D, et al.: Seroprevalence and risk factors of chikungunya virus infection in Mayotte, Indian Ocean, 2005–2006: a population-based survey. PLoS One 2008,3(8):e3066. 10.1371/journal.pone.0003066PubMedPubMed CentralView ArticleGoogle Scholar
- Hussain KM, Chu JJH: Insights into the interplay between chikungunya virus and its human host. Future Virol 2011,6(10):1211-1223. 10.2217/fvl.11.101View ArticleGoogle Scholar
- Ahola T, et al.: Critical residues of semliki forest virus RNA capping enzyme involved in methyltransferase and guanylyltransferase-like activities. J Virol 1997,71(1):392-397.PubMedPubMed CentralGoogle Scholar
- Gomez de Cedron M: RNA helicase activity of Semliki Forest virus replicase protein NSP2. FEBS Lett 1999,448(1):19-22. 10.1016/S0014-5793(99)00321-XPubMedView ArticleGoogle Scholar
- Vasiljeva L, et al.: Site-specific protease activity of the carboxyl-terminal domain of Semliki Forest virus replicase protein nsP2. J Biol Chem 2001,276(33):30786-30793. 10.1074/jbc.M104786200PubMedView ArticleGoogle Scholar
- Frolova E, et al.: Formation of nsP3-specific protein complexes during sindbis virus replication. J Virol 2006,80(8):4122-4134. 10.1128/JVI.80.8.4122-4134.2006PubMedPubMed CentralView ArticleGoogle Scholar
- Tomar S, et al.: Catalytic core of alphavirus nonstructural protein nsP4 possesses terminal adenylyltransferase activity. J Virol 2006,80(20):9962-9969. 10.1128/JVI.01067-06PubMedPubMed CentralView ArticleGoogle Scholar
- Sourisseau M, et al.: Characterization of reemerging chikungunya virus. PLoS Pathog 2007,3(6):e89. 10.1371/journal.ppat.0030089PubMedPubMed CentralView ArticleGoogle Scholar
- Ng LC, Hapuarachchi HC: Tracing the path of chikungunya virus–evolution and adaptation. Infect Genet Evol 2010,10(7):876-885. 10.1016/j.meegid.2010.07.012PubMedView ArticleGoogle Scholar
- Hapuarachchi HC, et al.: Re-emergence of chikungunya virus in south-east asia: virological evidence from Sri lanka and singapore. J Gen Virol 2010,91(Pt 4):1067-1076.PubMedView ArticleGoogle Scholar
- Staples JE, Breiman RF, Powers AM: Chikungunya fever: an epidemiological review of a re-emerging infectious disease. Clin Infect Dis 2009,49(6):942-948. 10.1086/605496PubMedView ArticleGoogle Scholar
- Powers AM, et al.: Re-emergence of chikungunya and O'nyong-nyong viruses: evidence for distinct geographical lineages and distant evolutionary relationships. J Gen Virol 2000,81(Pt 2):471-479.PubMedView ArticleGoogle Scholar
- Arankalle VA, et al.: Genetic divergence of chikungunya viruses in India (1963–2006) with special reference to the 2005–2006 explosive epidemic. J Gen Virol 2007,88(Pt 7):1967-1976.PubMedView ArticleGoogle Scholar
- Schuffenecker I, et al.: Genome microevolution of chikungunya viruses causing the Indian Ocean outbreak. PLoS Med 2006,3(7):e263. 10.1371/journal.pmed.0030263PubMedPubMed CentralView ArticleGoogle Scholar
- Vazeille M, et al.: Two chikungunya isolates from the outbreak of La reunion (Indian Ocean) exhibit different patterns of infection in the mosquito, aedes albopictus. PLoS One 2007,2(11):e1168. 10.1371/journal.pone.0001168PubMedPubMed CentralView ArticleGoogle Scholar
- Ng LC, et al.: Entomologic and virologic investigation of Chikungunya Singapore. Emerg Infect Dis 2009,15(8):1243-1249. 10.3201/eid1508.081486PubMedPubMed CentralView ArticleGoogle Scholar
- Leo YS, et al.: Chikungunya outbreak, Singapore, 2008. Emerg Infect Dis 2009,15(5):836-837. 10.3201/eid1505.081390PubMedPubMed CentralView ArticleGoogle Scholar
- Her Z, et al.: Active infection of human blood monocytes by chikungunya virus triggers an innate immune response. J Immunol 2010,184(10):5903-5913. 10.4049/jimmunol.0904181PubMedView ArticleGoogle Scholar
- Plaskon NE, Adelman ZN, Myles KM: Accurate strand-specific quantification of viral RNA. PLoS One 2009,4(10):e7468. 10.1371/journal.pone.0007468PubMedPubMed CentralView ArticleGoogle Scholar
- Khan AH, et al.: Complete nucleotide sequence of chikungunya virus and evidence for an internal polyadenylation site. J Gen Virol 2002,83(Pt 12):3075-3084.PubMedView ArticleGoogle Scholar
- Tsetsarkin KA, et al.: A single mutation in chikungunya virus affects vector specificity and epidemic potential. PLoS Pathog 2007,3(12):e201. 10.1371/journal.ppat.0030201PubMedPubMed CentralView ArticleGoogle Scholar
- Tsetsarkin KA, Weaver SC: Sequential adaptive mutations enhance efficient vector switching by chikungunya virus and its epidemic emergence. PLoS Pathog 2011,7(12):e1002412. 10.1371/journal.ppat.1002412PubMedPubMed CentralView ArticleGoogle Scholar
- Sumathy K, Ella KM: Genetic diversity of chikungunya virus, India 2006–2010: evolutionary dynamics and serotype analyses. J Med Virol 2012,84(3):462-470. 10.1002/jmv.23187PubMedView ArticleGoogle Scholar
- Park E, Griffin DE: The nsP3 macro domain is important for sindbis virus replication in neurons and neurovirulence in mice. Virology 2009,388(2):305-314. 10.1016/j.virol.2009.03.031PubMedPubMed CentralView ArticleGoogle Scholar
- Strauss JH, Strauss EG: The alphaviruses - gene-expression, replication, and evolution (Vol 58, Pg 496, 1994). Microbiol Rev 1994,58(4):806-806.Google Scholar
- Varjak M, Zusinaite E, Merits A: Novel functions of the alphavirus nonstructural protein nsP3 C-terminal region. J Virol 2010,84(5):2352-2364. 10.1128/JVI.01540-09PubMedPubMed CentralView ArticleGoogle Scholar
- Myles KM, et al.: Effects of an opal termination codon preceding the nsP4 gene sequence in the O'Nyong-nyong virus genome on anopheles gambiae infectivity. J Virol 2006,80(10):4992-4997. 10.1128/JVI.80.10.4992-4997.2006PubMedPubMed CentralView ArticleGoogle Scholar
- Grimley PM, Berezesky IK, Friedman RM: Cytoplasmic structures associated with an arbovirus infection: loci of viral ribonucleic acid synthesis. J Virol 1968,2(11):1326-1338.PubMedPubMed CentralGoogle Scholar
- Froshauer S, Kartenbeck J, Helenius A: Alphavirus RNA replicase is located on the cytoplasmic surface of endosomes and lysosomes. J Cell Biol 1988,107(6 Pt 1):2075-2086.PubMedView ArticleGoogle Scholar
- Kujala P, et al.: Biogenesis of the Semliki Forest virus RNA replication complex. J Virol 2001,75(8):3873-3884. 10.1128/JVI.75.8.3873-3884.2001PubMedPubMed CentralView ArticleGoogle Scholar
- Frolova EI, et al.: Functional sindbis virus replicative complexes are formed at the plasma membrane. J Virol 2010,84(22):11679-11695. 10.1128/JVI.01441-10PubMedPubMed CentralView ArticleGoogle Scholar
- Krejbich-Trotot P, et al.: Chikungunya virus mobilizes the apoptotic machinery to invade host cell defenses. FASEB J 2011,25(1):314-325. 10.1096/fj.10-164178PubMedView ArticleGoogle Scholar
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