Fig. 6

Inhibition of pro-inflammatory cytokine production in ASFV-infected PAMs. Effect of berbamine (Ber) or tetrandrine (Tet) on inhibition of (A) TNF-α, (B) IL-1β, and (C) IL-6 production in ASFV-infected cells at 24 h post-infection. (D-F) Corresponding measurements at 48 h post-infection. Cytokine measurements were performed by ELISA on collected supernatants. The concentration of berbamine and tetrandrine for the experiments were 50 µM and 25 µM, respectively. ASFV means virus-only control without compound addition while Ber and Tet denote berbamine and tetrandrine, respectively. Results represent the mean ± s.d. of three independent experiments (n = 3). Significant differences compared to other test groups are denoted by ^*p < 0.05, ^**p < 0.01, and ^***p < 0.001