Fig. 2

The virome analysis workflow for chunkung plants. Total RNA was extracted from each pooled sample. Libraries were prepared from the extracted RNA and sequenced using the Illumina HiSeq 4000 platform to generate the raw read data. Quality control was applied to obtain high-quality reads, and de novo assembly was used to reconstruct contigs from these reads. The contigs were aligned to the GenBank database for identification and comparison with known plant viral sequences. To confirm the presence of the viral species, primers were designed from the viral sequence fragments and validated by (RT-) PCR. Sanger sequencing was conducted to verify the obtained amplicons, and interactive visualization was performed. This comprehensive workflow involved sample collection, RNA extraction, library preparation, sequencing, data analysis, confirmation, and visualization for the virome analysis of chunkung plants