Table 1 Sequences of used primers and probes Probe modification: 5′6-FAM and 3′BHQ1
|  | Gene | Forward primer sequence (5′ to 3′) | Reverse primer sequence (5′ to 3′) | Probe sequence (5′ to 3′) |
|---|---|---|---|---|
RT-qPCR | GPX4 | AAGTAAACTACACTCAGCTCGTC | AAACCACACTCAGCGTATCGG | Â |
| Â | STING | TAACCTGAGTATGGCTGACCCCAA | CTGTAAACCCGATCCTTGATGC | Â |
|  | β-actin | CATGTACGTTGCTATCCAGGC | CTCCTTAATGTCACGCACGAT |  |
MethyLight | GPX4 | TCGACGGGTATATGGTTAATTTGGAT | GTTAATAACGATACACACGAAACCCCTA | CCAAACGAACGCCCACCGAT |
|  | β-actin | TGGTGATGGAGGAGGTTTAGTAAGT | AACCAATAAAACCTACTCCTCCCTTAAA | ACCACCACCCAACACACAATAACAAACACA |