Fig. 2
From: A macrophage-cell model of HIV latency reveals the unusual importance of the bromodomain axis
Reactivation of latently infected dTHP-1 single clones with JQ1. (A) Expression of csGFP in different clones harboring one copy of HIV. Established clones were differentiated for 48 h followed by incubation with JQ1, and csGFP (reactivation) was measured with FACS analysis. (B-D) A full range of HIV products is expressed upon reactivation of latent HIV in representative clone 55.2. Differentiated cells were reactivated with JQ1 for 48 h and HIV expression was assayed with microscopy (B), Western blot for HIV proteins (C), and quantitative RT PCR for gag mRNA (D). Percent of csGFP + cells is shown below the image in B. Data are means, and error bars indicate SEM (n = 3). *, p < 0.001, Student’s t test