Fig. 3

a Antigenic cartography using live SARS-CoV-2 viruses in BBB + BIV group (n = 8). MDS was used to create an antigenic map from the PRNT50 titers generated against WT, BA.5, XBB.1.5, XBB.1.16, XBB.1.9.1, XBB.2.3.2, EG.5.1, HK.3, BA.2.86, and JN.1 viruses on VeroE6-TMPRSS2 cells. In the map, coloured circles represent viruses, while squares denote antisera. The arrangement of viruses and antisera is such that their distances are inversely proportional to the antibody titers, minimizing error. The background grid corresponds to a twofold dilution of antisera in the titration process. Fold dilution differences between WT and omicron subvariants BA.5, XBB.1.5, XBB.1.16, XBB.1.9.1, XBB.2.3.2, EG.5.1, HK.3, BA.2.86, and JN.1 were 2.3, 5.5, 5.6, 5.7, 5.6, 6.1, 6.2, 4.8, and 6.7 respectively. b Antigenic cartography using live SARS-CoV-2 viruses in BBBB group(n = 18). MDS was used to create an antigenic map from the PRNT50 titers generated against WT, BA.5, XBB.1.5, XBB.1.16, XBB.1.9.1, XBB.2.3.2, EG.5.1, HK.3, BA.2.86, and JN.1 viruses on VeroE6-TMPRSS2 cells. In the map, coloured circles represent viruses, while squares denote antisera. The arrangement of viruses and antisera is such that their distances are inversely proportional to the antibody titers, minimizing error. The background grid corresponds to a twofold dilution of antisera in the titration process. Fold dilution differences between WT and omicron subvariants BA.5, XBB.1.5, XBB.1.16, XBB.1.9.1, XBB.2.3.2, EG.5.1, HK.3, BA.2.86, and JN.1 are 5.0, 7.1, 7.3, 7.2, 7.1, 7.5, 7.1, 6.9 and 7.4 respectively