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Fig. 3 | Virology Journal

Fig. 3

From: Development and application of an indirect ELISA for detection of antibodies against emerging atypical porcine pestivirus

Fig. 3

Evaluation of the E2-iELISA. A Determination of the cutoff value of the E2-iELISA. The cutoff value was determined by the formula (average S/P value + 3SD) using negative sera (n = 165) against APPV. Anti-APPV sera were served as positive controls. B Evaluation of the sensitivity of the E2-iELISA using serially diluted swine sera with known anti-APPV NAb titers. The numbers in brackets indicate the NAb titers. C Evaluation of the specificity of the E2-iELISA using a panel of swine antisera against serval non-APPV viruses, including BVDV (n = 4), PRV (n = 5), PCV2 (n = 3), PRRSV (n = 4), and CSFV (n = 4). D Evaluation of the diagnostic performance of the E2-iELISA by ROC curve analysis using MedCalc Software when detecting 70 negative and 120 positive sera against APPV verified with VNT

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Virology Journal

ISSN: 1743-422X