Fig. 2From: Development and application of an indirect ELISA for detection of antibodies against emerging atypical porcine pestivirusOptimization of working conditions of the E2-iELISA. A The checkerboard titration was performed to determine the optimal concentration of the coating antigen and serum dilution. B and C Optimization of the secondary antibody dilution and reaction time of TMB. D and E Optimization of the incubation time of sera and the secondary antibodyBack to article page