Fig. 1

Expression of the APPV E2 protein in mammalian suspension HEK293 cells. A Suspension HEK293 cells were transduced with the lentiviral particles containing the recombinant plasmid pLVX-APPV-E2. The green fluorescence of cells was used as an indicator of protein expression, and mock-transduced suspension HEK293 cells were used as a negative control. The scale bar is 400 μm. B Western blotting analysis of the recombinant E2 protein using anti-His-tag MAb. M, protein marker; lane 1, supernatants collected from suspension HEK293 cells transduced with the empty vector at 96 h; lane 2, HEK293-APPV-E2 cell line culture supernatant at 96 h post-transduction. C SDS-PAGE analysis of the purified recombinant E2 protein. M, protein marker; lane 1, supernatants collected from suspension HEK293 cells transduced with the empty vector; lane 2, culture supernatants of the recombinant suspension HEK293-APPV-E2 cell line; lane 3, purified recombinant E2 protein