Fig. 9

Photomicrograph sections from myelocytomatosis in liver, kidney, spleen, bone, and bone marrow of naturally infected broiler chickens. A Liver showing myeloid and lymphoid cell infiltrations (H&E, scale bar: 50 µm), B Liver showing lymphoblastic cells infiltration which are large, homogenous in size with poorly defined cytoplasmic membrane and basophilic cytoplasm (H&E, scale bar: 20 µm), C Kidney showing neoplastic aggregations of granular myeloid cells around renal tubules (arrow) and glomerulus (H&E, scale bar: 50 µm), D Nephrobastoma showing very dense fibrous capsule, the stromal tissue showed ill distinct glomeruli. Tumor tissue showed undifferentiated cystic renal tubules. The tubules are lined by multilayer epithelial cells (H&E, scale bar: 200 µm), E Spleen showing diffuse proliferation of large lymphoid cells in the red pulp (H&E, Scale bar: 50 µm), and F Spleen showing infiltration of myeloid cells in white pulp (arrow) (Giemsa stain, Scale bar: 20 µm). G Skull bone showing proliferation of granulated myelocytes in the bone marrow (arrow) (H&E, Scale bar: 200 µm), H Sternum bone showing proliferation of granulated myelocytes in the bone marrow (arrow) (Giemsa stain, Scale bar: 50 µm), I Myelocytoma; the cytoplasm of tumor cells were slightly basophilic and contained eosinophilic pinkish granules (Giemsa stain, Scale bar: 20 µm), and J Sternum bone showing proliferation of granulated myelocytes in the bone marrow (H&E, Scale bar: 50 µm)