Fig. 4

Protein structural analysis of Mac-2 specific amino acid substitutions within Nsp3 of SARS-CoV-2 from Pakistani Isolates. Comparison of 3-dimensional conformations of SARS-CoV-2 macrodomain-2 with their corresponding mutant versions. (a) Predicted structure of wild-type Mac-2 (YP_009725299.1). (b-d) Superimposed structure of wild-type Mac-2 (YP_009725299.1), and mutant versions of Mac-2 (T428I: QQH16492, V481L: EPI_ISL_1385798, and K487N: QNV71166). Descriptions of color codes are given in the panel. Deviated residues in terms of backbone torsion angles (Φ°, Ψ°) are shown in red color. Structural deviations are examined by RMSD values and given in the panel b, c, and d. Note. Primary sequence and secondary structural level details for comparisons in a–d is given in Additional File 1: Tables S5