Identification and characterisation of Gamma-herpesviruses in zoo artiodactyla

Table 2 Primer sequences employed for the Consensus Pan-herpes PCR according to Ehlers et al., 1999 [24]

FIRST-ROUND PCR
	*Forward*	*Reverse*
Degenerate primers	DFA: 5^’-GAYTTYGCNAGYYTNTAYCC-3^’ ILK: 5^’-TCCTGGACAAGCARNYSGCNMTNAA-3^’	KG1: 5^’-GTCTTGCTCACCAGNTCNCANCCYTT-3^’
Deoxyinosine- Substituted equivalent primers	DFA: 5^’-GAYTTYGCI^aAGYYTI^aTAYCC-3^’ ILK: 5^’-TCCTGGACAAGCARI^aYSGCI^aMTI^aAA-3^’	KG1: 5^’-GTCTTGCTCACCAGI^aTCI^aCAI^aCCYTT-3^’
Product 440 bp
SECOND-ROUND PCR
	Forward	Reverse
Degenerate primers	TGV: 5^’-TGTAACTCGGTGTAYGGNTTYACNGGNGT-3’	IYG: 5^’-CACAGAGTCCGTRTCNCCRTADAT-3’′
Deoxyinosine- Substituted equivalent primers	TGV: 5^’-TGTAACTCGGTGTAYGGI^aTTYACI^aGGI^aGT-3’	IYG: 5^’-CACAGAGTCCGTRTCI^aCCRTAI^aAT-3’
Product 220 bp

Degenerate and inosine-substituted equivalents are shown in bold. Degenerate bases are coded according to the IUPAC system [25]. I = Deoxyinosine.

ISSN: 1743-422X