Table 1 Conditions and primers for PCR amplification of species, antibiotic resistance genes, and virulence markers in E. coli isolates
Virulence factor | Target gene | Primer | Sequence (5` to 3`) | Length (bp) | Annealing temperature (°C) | Amplicon Size (pb) | References |
|---|---|---|---|---|---|---|---|
traT | traT | traT-F traT-R | GGTGTGGTGCGATGAGCACAG CACGGTTCAGCCATCCCTGAG | 21 21 | 60 | 290 | [26] |
fimH | fimH | fimH-F fimH-R | CATTCGCCTGTAAAACCGCC ATAACACGCCGCCATAAGCC | 20 20 | 60 | 207 | [27] |
blaCTX | blaCTX | blaCTX-F blaCTX-R | TTTGCGATGTGCAGTACCAGTAA CGATATCGTTGGTGGTGCCATA | 23 23 | 55 | 544 | [28] |
tetA | tetA | tetA-F tetA-R | TTGGCATTCTGCATTCACTC GTATAGCTTGCCGGAAGTCG | 20 20 | 60 | 494 | [29] |
blaTEM | blaTEM | TEM-F TEM-R | ATAAAATTCTTGAAGACGAAA GACAGTTACCAATGCTTAATCA | 19 19 | 50 | 1150 | [30] |
blaSHV | blaSHV | SHV-F SHV-R | CACTCAAGGATGTATTGTG TTAGCGTTGCCAGTGCTCG | 19 19 | 50 | 885 | [31] |