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Fig. 5 | Virology Journal

Fig. 5

From: Impaired influenza A virus replication by the host restriction factor SAMHD1 which inhibited by PA-mediated dephosphorylation of the host transcription factor IRF3

Fig. 5

Effect of H5N1 on phosphorylation of SAMHD1. A549 cells were infected with H5N1 at MOIs of 0.5 (A) and 1.5 (B), respectively. The cells were harvested at the indicated time points.IB analysis of H5N1 NP, SAMHD1and SAMHD1-P in cells was performed, with GAPDH as a loading control (A, B). The expression levels of SAMHD1 protein were quantified with ImageJ software to calculate the values relative to that for GAPDH. SAMHD1 RNA levels in cell lysates were detected by RT‒qPCR and normalized to GAPDH, and the RNA level in A549 cells at 0 h post infection was set as 1 (C). Data are the mean ± SD of three independent experiments. The results are representative of those from three independent repeats (mean ± SD, *p < 0.05, **p < 0.01, paired t test)

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Virology Journal

ISSN: 1743-422X