Fig. 6
From: ZNF148 inhibits HBV replication by downregulating RXRα transcription
ZNF148 is responsible for regulating the transcription and replication of HBV in vivo. (A) A mouse model of HBV infection was established by delivering prcccDNA and Cre plasmids into C57BL/6 mice via high-pressure distal intravenous injection (HDI). Subsequently, successfully infected mice were randomly allocated to the AAV-EGFP group (n = 8) or the AAV-ZNF148 group (n = 8). (B) Real-time PCR was used to measure the level of HBV core DNA in the serum of the mice (C-D), and the levels of HBV RNAs in the liver were measured using real-time PCR. (E) The level of HBV core DNA in the mouse liver was quantified using real-time PCR. (F) The expression of the HBc protein in the mouse liver was determined via Western blot analysis. (G-H) The serum concentrations of HBsAg and HBeAg were measured via ELISA. (I) Immunohistochemistry was carried out to examine the expression of HBs and HBc in the mouse liver. *P < 0.05, **P < 0.01