Fig. 5
Effect of PRV on apoptosis of porcine granulosa cells. Apoptosis level was measured in porcine granulosa cells infected with PRV at titers 5, 10, 50 TCID50 or Mock using flow cytometry. (A) Representative image of flow cytometry-based analysis. (B) Quantitative analysis of the apoptotic cells stained as Annexin V-FITC positive. (C and D) Expression of apoptosis-related proteins including cleaved-caspase 9, caspase 9, Bax and Bcl-2 was determined by Western blotting. Data were shown as mean ± SEM values of three independent experiments. Significant differences between Mock- and PRV-infection are indicated by * P < 0.05, ** P < 0.01