Fig. 2

PRV infection inhibited cell viability and Ki67 level in porcine ovarian granulosa cells. Porcine granulosa cells were infected with PRV suspension (5, 10 and 50 TCID₅₀) for the indicated time points. (A) Cell viability was detected by MTS assay. Data represented as mean ± SEM (n = 3); Asterisks indicate a significant difference compared to the Mock group. **P < 0.01. (B) PRV infection (50 TCID₅₀) caused obvious cytopathic effects in granulosa cells at 24 h post infection. (C) Expression of Ki67 in granulosa cells following PRV infection at 36 h by IFA. (D) Quantification of the fluorescence signal by Image J. Data show as mean ± SEM (n = 3); * P < 0.05, ** P < 0.01