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Table 2 Primers for inserting the E6 or E7 segments into plasmid pMCSG19 using ‘In-fusion’ PCR

From: Production of recombinant HPV11/16 E6/E7-MBP-His6 fusion proteins and their potential to induce cytokine secretion by immune cells in peripheral blood

Gene

Sequence of forward primer (5′→3′)

Sequence of reverse primer(5′→3′)

HPV11 E6

AGAACCTGTACTTCCAATCCATGGAAAGTAAAGATGCCT

CAGTGGTGGTGGTGGTGGTGGGGTAACAAGTCTTCCATG

HPV11 E7

AGAACCTGTACTTCCAATCCATGCATGGAAGACTTGTTA

CAGTGGTGGTGGTGGTGGTGTGGTTTTGGTGCGCAGATG

HPV16 E6

AGAACCTGTACTTCCAATCCATGCACCAAAAGAGAACTGC

CAGTGGTGGTGGTGGTGGTGCAGCTGGGTTTCTCTACGTG

HPV16 E7

AGAACCTGTACTTCCAATCCATGCATGGAGATACACCTAC

CAGTGGTGGTGGTGGTGGTGTGGTTTCTGAGAACAGATGG

  1. The underlined sections represented homologous sequences complementary to the plasmid vector insertion site, which were added to the 5′ ends of the forward primers and reverse primers of the target genes.
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Virology Journal

ISSN: 1743-422X