Table 1 Primers used for qPCR and RT-qPCR
Virus | Target | Primer pair | Final conc. (µM) | References |
|---|---|---|---|---|
EhV | Major capsid protein gene | F: 5'-TTCGCGCTCGAGTCGATC-'3 | 0.7 | [41] |
R: 5'-GACCTTTAGGCCAGGGAG-3' | ||||
ASFV | p72 gene | F: 5'-AGGAGGTATCGGTGGAGGGAAC-3' | 0.35 | This study |
R: 5'-ATGTCCAGATACGTTGCGTCCG-3' | ||||
PRRSV | GP7-3'UTR region | F: 5'-TAAGTTAYACTGTGGAGTTYAG-3' | 0.25 | This study |
R: 5'-CGCCCTAATTGAATAGGTGACTTAG-3' |