Fig. 3
Inactivation kinetics of EhV, ASFV and PRRSV. A S-qPCR and B V-qPCR results of EhV-86 (titer 1 × 106 μL−1) exposed to temperatures ranging from 4 to 100 °C for 20 min. C V-qPCR results of ASFV (titer 1 × 105 μL−1) exposed to temperatures of 4 °C, 60 °C, 80 °C and 100 °C for 20 min. D V-RT-qPCR results of PRRSV (103 TCID50 μL−1) exposed to temperatures of 4 °C, 60 °C and 100 °C for 20 min. In the absence of genomic standards for PRRSV, Cq data was converted to TCID50 units as described in the Material and Methods. Mean virus counts and SD from three biological replicates shown as measured by S-qPCR or S-RT-qPCR (light grey) and V-qPCR or V-RT-qPCR using 100 µM PMAxx final conc. (dark grey). Asterisks represent differences between control and treated samples by ANOVA (Tukey’s test) at 95% confidence level