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Fig. 4 | Virology Journal

Fig. 4

From: Preclinical assessment of an anti-HTLV-1 heterologous DNA/MVA vaccine protocol expressing a multiepitope HBZ protein

Fig. 4

Analysis of HBZ multiepitope protein expression by the pcDNA and MVA vector. A Representative graphs of the gating strategy used in the flow cytometry expression assays. Total percentage of HBZ expression in BHK-21 transfected / infected cells. Subset low (subset low), subset (subset medium) and subset high (subset high) according to cell size (FSC) and granularity (SSC). A1 for pcDNA3.1(+)-HBZ and A2 for MVA-HBZ. B–B1 In black: pcDNA3.1(+)-HBZ; In gray: Mock transfected cell control. B2 In black: MVA-HBZ; In gray: MVA-GFP control. Both vectors showed efficient recombinant protein expression in vitro mainly in subsets medium and high

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Virology Journal

ISSN: 1743-422X