Fig. 1

Schematic diagram of HTLV-1 proviral genome and the HBZ multiepitope cassette construction. A HTLV-1 proviral genome pointing out the HTLV-1 basic leucine zipper factor (HBZ) in the antisense strand. The sHBZ box demonstrate in the Activator Domain (AD) and bZIP region the position of peptides 1 to 3 (in silico analysis), peptide 4 (MacNamara et al. [23]), and peptide 5 (Sugata et al. [17]) that compound the multiepitope recombinant protein. B HBZ multiepitope cassette presenting the selected peptides (pep1 to pep5), spacer regions (GSGSG), signal peptide (MDAMKRGLCCVLLLCGAVFVDSVTG), and a flag tag (DYKDDDDK). C Construction of recombinant MVA-HBZ by homologous recombination expressing the HBZ multiepitope protein. A pLW44 plasmid that contains the gene of interest is used to transfect an MVA-infected cell. The pcDNA3.1(+)-HBZ express the same HBZ multiepitope protein