Table 1 Oligonucleotides used in this experiment
From: Rapid detection of avian influenza virus based on CRISPR-Cas12a
Primer name | Sequence (5′–3′) | Product size (bp) |
|---|---|---|
crRNA-M-1 | UAAUUUCUACUAAGUGUAGAUAAGAAAAGACGAUCAAGAAUCC | Â |
crRNA-M-2 | UAAUUUCUACUAAGUGUAGAUCAGGCCUACCAGAAACGGAU | Â |
crRNA-M-3 | UAAUUUCUACUAAGUGUAGAUCACUCCCATCCGUUUCUGG | Â |
crRNA-M-4 | UAAUUUCUACUAAGUGUAGAUUGUUCACGCUCACCGUGCCCAG | Â |
mRPA-4-F1 | CAAGACCAATC CTGTCACCTCTGACTAAGGG G | 103 |
mRPA-4-R1 | TTTTGGACAAAGCGTCTACGCTGCAGTCCTCGCTC | Â |
mRPA-4-F2 | CAATC CTGTCACCTCTGACTAAGGGGATTTTAGGG | 97 |
mRPA-4-R2 | TTTTGGACAAAGCGTCTACGCTGCAGTCCTCGCTC | Â |
mRPA-4-F3 | AGATCG CGCAGAGACTTGAGGATGTCTTTGCAGGG | 214 |
mRPA-4-R3 | TCCATGTTGTTTGGGTCTCCATTTCCATTTAGGGC | Â |
crRNA-NP-1 | UAAUUUCUACUAAGUGUAGAUCGUCUGCUUCAAAACAGCCAG | Â |
NP1-RPA-F1 | TGGATATGACTTTGAGAGAGAAGG GTACTCCCTGG | 137 |
NP1-RPA-R1 | ATGCCATCCACACTAGTTGACTCTTGTGTGCTGGG | Â |
NP1-RPA-F2 | TATGACTTTGAGAGAGAAGGGTACTCCCTGGTTGG | 212 |
NP1-RPA-R2 | GATAGCTGTCCTCTTGGGACCATTCTTGTCCCTC | Â |
NP1-RPA-F3 | GAGAGAGAAGGGTACTCCCTGGTTGGAATAGATCC | 89 |
NP1-RPA-F3 | TTCTCATTTGGTCTAATGAGACTAAAGACCTGGC | Â |
crRNA-NP-2 | UAAUUUCUACUAAGUGUAGAUCCGGAGAAGAGACGGGAAAUGG | Â |
crRNA-NP-3 | UAAUUUCUACUAAGUGUAGAUUGGCAAGGUCUGCACUCAUCCU | Â |
crRNA-NP-4 | UAAUUUCUACUAAGUGUAGAUGAAUUUCCCUUUGAGGAUGUUGC | Â |