Fig. 5

Sensitivity analysis. a, c, e Ten-fold serial dilutions of plasmid template targeting the M gene at 2.4 × 10¹¹ copies/µl for the sensitivity assay; b, d, f tenfold serial dilutions of plasmid template targeting the NP gene at 8.67 × 10¹¹ copies/µl for the sensitivity assay; (M:11–4, 2.4 × 10¹¹ copies/µl–2.4 × 10⁴ copies/µl; NP:11–4, 8.67 × 10¹¹ copies/µl–8.67 × 10⁴ copies/µl); g, h, i, m tenfold serial dilutions of RNA template targeting M gene at 6.7 × 10¹¹ copies/μL for the sensitivity assay by RT-RPA/CRISPR; i, k, l, n tenfold serial dilutions of RNA template targeting NP gene at 12 × 10¹¹ copies/μL for the sensitivity assay by RT-RPA/CRISPR. (M:11–0, 6.7 × 10¹¹ copies/µl–6.7 × 10⁰ copies/µl; NP:11–0, 12 × 10¹¹ copies/µl–12 × 10⁰ copies/µl). Fluorescent signals were collected every 5 min and displayed for 2 h and 30 min, respectively