Fig. 4
From: Rapid detection of avian influenza virus based on CRISPR-Cas12a
Design and screening of crRNA and RPA primers. a Cleavage activity of CRISPR/Cas12a induced by four crRNAs targeting the AIV M gene, b primer screening for the M gene, c cleavage activity of CRISPR/Cas12a induced by four crRNAs targeting the AIV NP gene, and d primer screening for the NP gene. A comprehensive screen using a random combination of primers was used to identify the primer pairs with the best performance. Fluorescence signals were collected using the QuantStudio software (Applied Biosystems)