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Fig. 3 | Virology Journal

Fig. 3

From: Rapid detection of avian influenza virus based on CRISPR-Cas12a

Fig. 3

Optimization of the RT-RPA and CRISPR/Cas12a system. a Cleavage effect of reaction buffers with different Mg2+ concentrations on the CRISPR/Cas12a assay. b Cleavage effect of different dilution concentrations of crRNA on Cas12a protein cleavage efficiency. c Cleavage effect of reaction buffers with different pH values on the CRISPR/Cas12a assay. d Effect of RNase H nuclease on RT-RPA assays

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Virology Journal

ISSN: 1743-422X