Fig. 1
From: Rapid detection of avian influenza virus based on CRISPR-Cas12a
Schematic diagram of the RT/RPA assay coupled with the CRISPR-mediated detection platform for identification of AIV. Target genes are specifically amplified by RT-RPA from genomic fragments isolated from clinical samples. A specific crRNA is designed to recognize the target gene and form a complex with Cas12a and the probe. Once the target DNA is recognized by the Cas12a and crRNA complex, Cas12a exerts its non-specific endonuclease activity and cleaves the single-stranded DNA probe. By introducing a ssDNA probe that labels the fluorophore and quencher or fluorophore and biotin, the cleavage could be observed using a fluorophore reader or lateral flow dipstick