Virology Journal

Table 3 HDA/RPA-LFT and q-PCR specific assays

From: Detection of monkeypox virus using helicase dependent amplification and recombinase polymerase amplification combined with lateral flow test

Puedovirus^a	HDA-LFT		Total		RPA-LFT		Total		qPCR		Total
Puedovirus^a	Positive	Negative	Total		Positive	Negative	Total		Positive	Negative	Total
Positive^b	19	1	20		18	2	20		20	0	20
Negative^c	3	17	20		1	19	20		2	18	20
Total	22	18	40		19	21	40		22	18	40
Sensitivity	86%				95%				91%
Specificity	94%				90%				100%
Accuracy	90%				93%				95%

^a Samples containing nucleic acid extracts of MPX PV and other viruses of the same genus
^b Simulated clinical samples containing only MPX PV nucleic acid
^c Simulated clinical samples without MPX PV nucleic acid (HS PV, VZ PV, CPX PV).

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ISSN: 1743-422X

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