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Table 2 Sensitive detection by HDA/RPA-LFT and qPCR assay

From: Detection of monkeypox virus using helicase dependent amplification and recombinase polymerase amplification combined with lateral flow test

Total

HDA-LFT

 

RPA-LFT

 

qPCR

Positive

Copies

(copies/µL)

Positive detection rate

 

Positive

Copies

(copies/µL)

Positive detection rate

 

Positive

Copies

(copies/mL)

Positive detection rate

20

20

1000

100%

 

20

1000

100%

 

20

5000

100%

20

20

100

100%

 

20

100

100%

 

19

500

95%

20

19

10

95%

 

20

10

100%

 

6

50

30%

20

4

1

20%

 

2

1

10%

 

1

5

5%

20

1

0

5%

 

1

0

5%

 

0

0

0%

  1. Sensitive detection by HDA/RPA-LFT and qPCR assay