Detection of monkeypox virus using helicase dependent amplification and recombinase polymerase amplification combined with lateral flow test

Table 1 Sequences of primers and probe for HDA/RPA-LFT and qPCR assay

Primer/Probe^a	Sequence(5′-3′)	Fluorophore/quencher	Fragment length
4HDA-LFT-F	TTAATAACGGTGACAGGGTTAACACC	5’-Dig 5’-Biotin	102 bp
4HDA-LFT-R	CCGCGAAAAATCAATGAGAGAG	5’-Dig 5’-Biotin	102 bp
4RPA-LFT-F	CTGCCTTATCGAATACTCTTCCGTCAATGTCTA/	5’-Dig 5’-Biotin	138 bp
4RPA-LFT-F	internal spacer/ACAGGCATAAAATGT-carbon spacer^b
4RPA-LFT-R	ACCCTGTCACCGTTATTAATGAGTACTGTC
2qPCR-F	GCCCCACTGATTCAATACGAA		83 bp
2qPCR-R	AGGTGTTAACCCTGTCACCGTTA
2qPCR-P	AGACCAATCTCTCCTAGTTAT	5’ 6-FAM/3’-BHQ1

ISSN: 1743-422X