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Fig. 5 | Virology Journal

Fig. 5

From: Detection of monkeypox virus using helicase dependent amplification and recombinase polymerase amplification combined with lateral flow test

Fig. 5

qPCR reaction optimization. The graph shows the optimisation of the qPCR reaction, where graph a show the optimisation of primer concentrations, from left to right, with a gradient of 2µM 12µM and the NC results. Figure b shows the optimisation of probe concentrations, from left to right, with a gradient of 2µM -12µM and the NC results. Figure c shows the optimisation of the annealing temperature, from left to right, with a gradient of 50-58 °C and the NC results

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