Fig. 4

RPA-LFT reaction optimisation. The graph shows the optimisation of the RPA-LFT reaction, where graph a show the optimisation of the reaction time, from left to right, with a gradient of 10 − 30 min, and shows the NC results respectively. Figure b shows the optimisation of the reaction temperature, from left to right, with a gradient of 26-38 °C, and shows the NC results respectively. Figure c shows the optimisation of primer concentrations, from left to right, with a gradient of 2µM -12µM, and NC results respectively. Figure d shows the optimisation of MgOAc, from left to right, for 160 mM -280 mM and NC results respectively. Figure e shows the optimisation of Nfo, from left to right, for 0.04 mg/mL -0.12 mg/mL and NC results, respectively. PC stands for positive control