Skip to main content
Fig. 3 | Virology Journal

Fig. 3

From: Detection of monkeypox virus using helicase dependent amplification and recombinase polymerase amplification combined with lateral flow test

Fig. 3

HDA-LFT reaction optimisation. The figure shows the optimisation of the HDA-LFT reaction, where graph figure a show the optimisation of the reaction time, from left to right, with a gradient of 35 − 75 min and the negative control (NC) results respectively. Figure b shows the optimisation of the reaction temperature, from left to right, with a gradient of 45-65 °C and the NC results respectively. Figure c shows the optimisation of primer concentrations, from left to right, with a gradient of 2µM (µmol/L) -12µM and the NC results respectively. Figure d shows the optimisation of dNTPs, from left to right, for 50µmol/L -130µmol/L and the NC results respectively

Back to article page

Virology Journal

ISSN: 1743-422X