CRISPR–Cas system to discover host-virus interactions in Flaviviridae

Table 2 Antiviral host factors identified by CRISPR screens

Virus	CRISPR screen	Cell type	CRISPR target genes	References
ZIKV	CRISPRa	Huh7	TMEM120A	[77]
	CRISPRa	Huh7.5	IFI6, IFN-λ2	[79]
	CRISPR KO	Human pluripotent stem cell (hPSC)-derived neural progenitors (NPs)	ISG15, SOCS3, STAT3	[80]
		A549	RIG-I, MDA5, IFNAR	[82]
		A549	OAS3, RNAseL, STAT1, STAT2, MAVS	[85]
DENV	CRISPR KO	A549	OAS3, RNAseL, STAT1, STAT2, MAVS	[85]
WNV	CRISPR KO	A549	OAS1, OAS2, OAS3, RNAse L	[83]
YFV and other flaviviruses	CRISPR KO	Huh7.5	IFI6, HSPA5	[49]
HCV	CRISPR KO	Huh7.5	STAT1, STAT2, IRF9, STAT3, STAT6	[84]

CRISPR clustered regularly interspaced short palindromic repeats, CRISPRi CRISPR interference, CRSPRa CRISPR activation, CRISPR-KO CRISPR knockout, DENV dengue virus, ZIKV Zika virus, YFV yellow fever virus, WNV West Nile virus, HCV hepatitis C virus, TMEM transmembrane protein, IFN interferon, ISG interferon-stimulated gene, SOCS suppressor of cytokine signaling, RIG retinoic acid-inducible gene, IFI interferon alpha inducible protein, MDA melanoma differentiation-associated protein, OAS oligoadenylate synthetase, MAVS mitochondrial antiviral signaling protein, HSPA heat shock protein family A

ISSN: 1743-422X