CRISPR–Cas system to discover host-virus interactions in Flaviviridae

Table 1 Proviral host factors identified utilizing CRISPR screens

Type of genetic screen	Virus	Cell line	Top screening hits	References
CRISPR KO	DENV	Huh7.5.1	OST complex (STT3A and STT3B), TRAP complex (SSR1, SSR2, SSR3), ERAD (SEL1L, AUP1, DERL2)	[14]
		Huh7.5.1	MAGT1	[50]
		Huh7.5.1, HEK293FT	EMC	[51]
		HAP1	DPM1 and DPM3	[52]
		HAP1	RACK1	[53]
	ZIKV	H1-HeLa	EMC, AXL, STT3A, TRAP complex	[54]
		HAP1	TMEM41B and VMP1	[55]
		Huh7.5	RACK1	[56]
	WNV	293FT	EMC2, EMC3, SEL1L, DERL2, UBE2G2, UBE2J1, HRD1	[57]
	WNV	293T	STTA3, SPCS1, SPCS3, SEC63	[58]
	YFV	HAP1	TMEM41B and VMP1	[55]
	HCV	Huh7.5	CD81, OCLN, CLDN1	[59, 60]
	HCV	Huh7.5.1	ELAVL1, RFK, FLAD1	[14]
CRISPRi	HCV	Huh7.5	TRIM26	[61]
CRISPRa	ZIKV	A549,SNB-19	RhoV, WWTR1	[62]

CRISPR clustered regularly interspaced short palindromic repeats, CRISPRi CRISPR interference, CRSPRa CRISPR activation, CRISPR-KO CRISPR knockout, DENV dengue virus, ZIKV zika virus, YFV yellow fever virus; WNV West Nile virus, HCV hepatitis C virus, OST Oligosaccharyltransferase, TRAP translocon-associated protein, ERAD endoplasmic reticulum-associated degradation, MAGT magnesium transporter, EMC ER membrane complex, DPM dolichol-phosphate mannose, RACK1 receptor for activated protein C kinase 1, TMEM41B transmembrane protein 41B, VMP1 vacuole membrane protein 1, SPC signal peptidase complex, UBE2J1 ubiquitin-conjugating enzyme E2 J1, RFK riboflavin kinase, OCLN occluding, FLAD1 flavin adenine dinucleotide synthetase 1, TRIM26 tripartite motif containing 26, WWTR1 WW domain containing transcription regulator 1, RhoV Ras homolog family member V

ISSN: 1743-422X