Fig. 6
From: Bardoxolone methyl inhibits the infection of rabies virus via Nrf2 pathway activation in vitro
CDDO-Me inhibited different RABV strains via the Nrf2-dependent signaling pathway in N2a cells. N2a cells were pretreated with ATRA (10 µM) for 6 h, infected with SC16 A, CVS-11 D or CTN G at an MOI of 10 and treated with CDDO-Me for 24 h. Virus-infected cells that were treated with DMSO served as a control. Nrf2, HO-1 and NQO1 levels were examined by western blotting. Data are representative of at least three independent experiments. B, E, H The results of quantitative analyses of the relative signal densities of Nrf2, HO-1 and NQO1 after normalization to the β-actin signal density at the indicated times are shown on the right. Each test was performed in triplicate. C, F, I Culture supernatants were sampled, and the viral titers of the culture supernatants were measured. Graphical data denote the mean ± SD. Statistical significance was assessed using one-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001